Identifying as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy.
BK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We...
| Publicado en: | Journal of Immunology Research pp. 1 - 15 |
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| Autores principales: | , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
7/31/2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=158264847&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 158264847 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23148861 GZMW jtl: Journal of Immunology Research issn: 23148861 maglogo: N pubinfo: dt: 7/31/2022 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 158264847 158264847 NLM35958876 158264847 10.1155/2022/6934744 NLM35958876 158264847 ppf: 1 ppct: 14 formats: tig: atl: Identifying as a Promising Diagnostic Biomarker for BK Virus-Associated Nephropathy. aug: au: Wang, Yicun Wang, Yuxuan Zhang, Di Zhang, Hao Wang, Wei Hu, Xiaopeng affil: Department of Urology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China sug: subj: Kidney Transplantation Adverse Effects Viruses Kidney Diseases Etiology Tumor Virus Infections Diagnosis Kidney Diseases Diagnosis Nephritis, Interstitial Polyomavirus Infections Diagnosis Proteins Graft Rejection ab: BK virus-associated nephropathy (BKVN) remains a major infectious complication due to powerful immunosuppression in kidney transplant recipients, and its histologic appearance can mimic rejection, leading to diagnostic and treatment dilemmas thus molecular diagnostic methods would be beneficial. We collected gene expression profiles of 169 kidney biopsies taken from BKVN, rejection, and stable functioning allografts, based on single sample gene set enrichment analysis and random forest algorithm, and three hallmark activities associated with DNA damage and proliferation were found to be relatively specific in BKVN. Subsequently, weighted gene co-expression network analysis and support vector machines (SVM) algorithm identified RBBP7 as a robust and promising biomarker with high accuracy in both training and validation cohorts (AUC =0.938, 0.977, respectively). Besides, potential drugs for BKVN treatment such as mepacrine were discovered, which may contribute to targeted antiviral therapy and effective patient management rather than simply reducing the doses of immunosuppressive agents in clinical practice. RBBP7 (retinoblastoma binding protein 7) serves as component of serval complexes that regulate chromatin metabolism and functions in affecting DNA replication and controlling cell proliferation. In this research, upregulation of RBBP7 was found to be associated with the higher infiltration of CD8 naïve T, iTreg, and neutrophil cells and the lower amounts of Th1, central memory T, NKT, CD8 T, and dendritic cells. Moreover, the infiltration of Th1, Th17, and NKT cells was steadily different between BKVN and rejection allografts through immune cell assessment. In conclusion, we identified and verified RBBP7 as a molecular biomarker for BKVN diagnosis, which demonstrated great distinguishing ability with allograft rejection and would support clinical decision-making. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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