Modeling and simulation of diffusion and reaction processes during the staining of tissue sections on slides.
Histological slides are an important tool in the diagnosis of tumors as well as of other diseases that affect cell shapes and distributions. Until now, the research concerning an optimal staining time has been mainly done empirically. In experimental investigations, it is often not possible to stain...
| Publicado en: | Histochemistry & Cell Biology Vol. 158; no. 2; pp. 137 - 149 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Aug2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=158278278&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 158278278 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 09486143 NQI jtl: Histochemistry & Cell Biology issn: 09486143 maglogo: N pubinfo: dt: Aug2022 vid: 158 iid: 2 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 158278278 157278488 10.1007/s00418-022-02118-9 158278278 ppf: 137 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Modeling and simulation of diffusion and reaction processes during the staining of tissue sections on slides. aug: au: Menning, Johannes D. M. Wallmersperger, Thomas Meinhardt, Matthias Ehrenhofer, Adrian affil: Technische Universität Dresden, Institute of Solid Mechanics, George-Bähr-Straße 3c, 01069, Dresden, Germany sug: ab: Histological slides are an important tool in the diagnosis of tumors as well as of other diseases that affect cell shapes and distributions. Until now, the research concerning an optimal staining time has been mainly done empirically. In experimental investigations, it is often not possible to stain an already-stained slide with another stain to receive further information. To overcome these challenges, in the present paper a continuum-based model was developed for conducting a virtual (re-)staining of a scanned histological slide. This model is capable of simulating the staining of cell nuclei with the dye hematoxylin (C.I. 75,290). The transport and binding of the dye are modeled (i) along with the resulting RGB intensities (ii). For (i), a coupled diffusion–reaction equation is used and for (ii) Beer–Lambert's law. For the spatial discretization an approach based on the finite element method (FEM) is used and for the time discretization a finite difference method (FDM). For the validation of the proposed model, frozen sections from human liver biopsies stained with hemalum were used. The staining times were varied so that the development of the staining intensity could be observed over time. The results show that the model is capable of predicting the staining process. The model can therefore be used to perform a virtual (re-)staining of a histological sample. This allows a change of the staining parameters without the need of acquiring an additional sample. The virtual standardization of the staining is the first step towards universal cross-site comparability of histological slides. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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