Computer Image Analysis Reveals C-Myc as a Potential Biomarker for Discriminating between Keratoacanthoma and Cutaneous Squamous Cell Carcinoma.

The distinction between Keratoacanthoma (KA) and Cutaneous Squamous Cell Carcinoma (cSCC) is critical yet usually challenging to discriminate clinically and histopathologically. One approach to differentiate KA from cSCC is through assessing the immunohistochemical staining patterns of the three ind...

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Published in:BioMed Research International pp. 1 - 18
Main Authors: Fan, Xinyun, Niu, Xueli, Wu, Ze, Yao, Lu, Chen, Shirui, Wan, Wenyu, Huang, Bo, Qi, Rui-Qun, Zhang, Tao
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell 8/23/2022
Online Access:View this record in EBSCOhost
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      dt: 8/23/2022
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2022/3168503
        158677289
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        atl: Computer Image Analysis Reveals C-Myc as a Potential Biomarker for Discriminating between Keratoacanthoma and Cutaneous Squamous Cell Carcinoma.
      aug:
        au:
          Fan, Xinyun
          Niu, Xueli
          Wu, Ze
          Yao, Lu
          Chen, Shirui
          Wan, Wenyu
          Huang, Bo
          Qi, Rui-Qun
          Zhang, Tao
        affil: Key Laboratory of Immunodermatology, Ministry of Education, Department of Dermatology, The First Hospital of China Medical University, Shenyang 110001, China
      sug:
        subj:
          Image Processing, Computer Assisted Utilization
          Oncogenes Evaluation
          Tumor Markers, Biological Analysis
          Keratoacanthoma Diagnosis
          Cutaneous Squamous Cell Carcinoma Diagnosis
          Human
          Immunohistochemistry Methods
          Cytoskeletal Proteins Analysis
          Staining and Labeling Methods
          Sensitivity and Specificity
      ab: The distinction between Keratoacanthoma (KA) and Cutaneous Squamous Cell Carcinoma (cSCC) is critical yet usually challenging to discriminate clinically and histopathologically. One approach to differentiate KA from cSCC is through assessing the immunohistochemical staining patterns of the three indicators, β-catenin, C-Myc, and CyclinD1, which are critical molecules that play important roles in the Wnt/β-catenin signaling pathway. Ki-67, as a proliferation biomarker for human tumor cells, was also assessed as an additional potential marker for differentiating KA from cSCC. In this report, these four indicators were analyzed in 42 KA and 30 cSCC cases with the use of the computer automated image analysis system. Computer automated image analysis is a time-based and cost-effective method of determining IHC staining in KA and cSCC samples. We found that C-Myc staining was predominantly localized in the nuclei of basal cells within KA patients, whereas cSCC staining was predominantly localized in the nuclei of diffuse cells. This C-Myc staining pattern has a sensitivity of 78.6% and a specificity of 66.7% for identifying KA. Moreover, positive rates of distinct expression patterns of C-Myc and Ki-67 may also serve as a means to clinically distinguish KA from cSCC. Taken together, our results suggest that these markers, in particular C-Myc, may be useful in differentiating KA from cSCC.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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