Temporal Variability in Inflammatory Gene Methylation and Delirium in Critically Ill Patients.

Background: Intensive care unit (ICU) delirium is associated with a proinflammatory state and poor outcomes. An epigenetic mechanism may modify inflammation. Objective: To identify inflammatory gene methylation trajectory groups and explore their clinical and demographic variability. Methods: Patien...

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Published in:American Journal of Critical Care Vol. 31; no. 5; pp. 367 - 375
Main Authors: Alexander, Sheila A., Conley, Yvette P., Girard, Timothy D., McVerry, Bryan J., Ren, Dianxu
Format: research Journal Article
Published: American Association of Critical-Care Nurses Sep2022
Online Access:View this record in EBSCOhost
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      jtl: American Journal of Critical Care
      issn: 10623264
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      dt: Sep2022
      vid: 31
      iid: 5
      pid: 2559
      pub: American Association of Critical-Care Nurses
      place: Alisa Veijo, California
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        158812731
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        10.4037/ajcc2022225
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        atl: Temporal Variability in Inflammatory Gene Methylation and Delirium in Critically Ill Patients.
      aug:
        au:
          Alexander, Sheila A.
          Conley, Yvette P.
          Girard, Timothy D.
          McVerry, Bryan J.
          Ren, Dianxu
        affil: Sheila A. Alexander is an associate professor of nursing, University of Pittsburgh, Pittsburgh, Pennsylvania
      sug:
        subj:
          Genes
          DNA Methylation Evaluation
          Inflammation
          Delirium
          Critically Ill Patients
          Sociodemographic Factors
          Human
          Adolescence
          Young Adult
          Adult
          Respiration, Artificial
          Confusion
          Intensive Care Units
          Structural Equation Modeling
          Descriptive Statistics
          White Persons
          Sex Factors
          Nonexperimental Studies
          Prospective Studies
          Female
          Male
          Data Analysis Software
          Chi Square Test
          Adolescent: 13-18 years
          Adult: 19-44 years
          Female
          Male
      ab: Background: Intensive care unit (ICU) delirium is associated with a proinflammatory state and poor outcomes. An epigenetic mechanism may modify inflammation. Objective: To identify inflammatory gene methylation trajectory groups and explore their clinical and demographic variability. Methods: Patients were at least 18 years old; received mechanical ventilation for at least 24 hours; had no brain disorder/injury, preexisting dementia, or positive toxicology screen; and were admitted to a medical or surgical/trauma ICU. Delirium was assessed (Confusion Assessment Method for the ICU) and blood samples were collected daily for up to 10 days. Methylation of 3 genes in the inflammatory pathway was quantified. Latent class analysis identified gene methylation trajectories, and variables (including delirium) were compared between trajectory groups. Results: Of 68 patients (53% female, 88% White), 65% developed delirium. Of 3 methylation trajectories for IL6ST, the group with low initial methylation increasing over time included younger male patients who were less likely to have delirium, and the group with high initial methylation decreasing over time included older (P =.01) female (P =.05) patients who more often had delirium (P =.05). IL17C had 2 methylation trajectories without significant differences in delirium, age, or sex. IL13RA1 had 2 methylation trajectories without differences in delirium or age; the group with sustained high methylation had more female patients (P =.003). Conclusions: Temporal variability in inflammatory gene methylation occurs after ICU admission. Delirium, female sex, and older age were more common with higher IL6ST methylation that decreased over time. Larger studies are needed to further elucidate these relationships.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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