AnFiSA: An open-source computational platform for the analysis of sequencing data for rare genetic disease.

Despite genomic sequencing rapidly transforming from being a bench-side tool to a routine procedure in a hospital, there is a noticeable lack of genomic analysis software that supports both clinical and research workflows as well as crowdsourcing. Furthermore, most existing software packages are not...

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Publicado en:Journal of Biomedical Informatics Vol. 133
Autores principales: Bouzinier, M.A., Etin, D., Trifonov, S.I., Evdokimova, V.N., Ulitin, V., Shen, J., Kokorev, A., Ghazani, A.A., Chekaluk, Y., Albertyn, Z., Giersch, A., Morton, C.C., Abraamyan, F., Bendapudi, P.K., Sunyaev, S., Undiagnosed Diseases Network, Brigham Genomic Medicine, SEQuencing a Baby for an Optimal Outcome, Quantori, Krier, J.B.
Formato: Journal Article
Publicado: Academic Press Inc. Sep2022
Acceso en línea:Ver este registro en EBSCOhost
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        atl: AnFiSA: An open-source computational platform for the analysis of sequencing data for rare genetic disease.
      aug:
        au:
          Bouzinier, M.A.
          Etin, D.
          Trifonov, S.I.
          Evdokimova, V.N.
          Ulitin, V.
          Shen, J.
          Kokorev, A.
          Ghazani, A.A.
          Chekaluk, Y.
          Albertyn, Z.
          Giersch, A.
          Morton, C.C.
          Abraamyan, F.
          Bendapudi, P.K.
          Sunyaev, S.
          Undiagnosed Diseases Network
          Brigham Genomic Medicine
          SEQuencing a Baby for an Optimal Outcome
          Quantori
          Krier, J.B.
        affil: Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA
      sug:
        subj:
          Genomics Methods
          Software
          Reproducibility of Results
          Management Information Systems
          Bioinformatics Methods
          Resource Databases
          Systems Analysis
          Multidimensional Health Locus of Control Scales
          Scales
      ab: Despite genomic sequencing rapidly transforming from being a bench-side tool to a routine procedure in a hospital, there is a noticeable lack of genomic analysis software that supports both clinical and research workflows as well as crowdsourcing. Furthermore, most existing software packages are not forward-compatible in regards to supporting ever-changing diagnostic rules adopted by the genetics community. Regular updates of genomics databases pose challenges for reproducible and traceable automated genetic diagnostics tools. Lastly, most of the software tools score low on explainability amongst clinicians. We have created a fully open-source variant curation tool, AnFiSA, with the intention to invite and accept contributions from clinicians, researchers, and professional software developers. The design of AnFiSA addresses the aforementioned issues via the following architectural principles: using a multidimensional database management system (DBMS) for genomic data to address reproducibility, curated decision trees adaptable to changing clinical rules, and a crowdsourcing-friendly interface to address difficult-to-diagnose cases. We discuss how we have chosen our technology stack and describe the design and implementation of the software. Finally, we show in detail how selected workflows can be implemented using the current version of AnFiSA by a medical geneticist.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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