Expert opinion on NSCLC small specimen biomarker testing - Part 2: Analysis, reporting, and quality assessment.

The diagnostic work-up for non-small cell lung cancer (NSCLC) requires biomarker testing to guide therapy choices. This article is the second of a two-part series. In Part 1, we summarised evidence-based recommendations for obtaining and processing small specimen samples (i.e. pre-analytical steps)...

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Publicado en:Virchows Archiv: European Journal of Pathology Vol. 481; no. 3; pp. 351 - 367
Autores principales: Penault-Llorca, Frédérique, Kerr, Keith M., Garrido, Pilar, Thunnissen, Erik, Dequeker, Elisabeth, Normanno, Nicola, Patton, Simon J., Fairley, Jenni, Kapp, Joshua, de Ridder, Daniëlle, Ryška, Aleš, Moch, Holger
Formato: research Journal Article
Publicado: Springer Nature Sep2022
Acceso en línea:Ver este registro en EBSCOhost
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          Penault-Llorca, Frédérique
          Kerr, Keith M.
          Garrido, Pilar
          Thunnissen, Erik
          Dequeker, Elisabeth
          Normanno, Nicola
          Patton, Simon J.
          Fairley, Jenni
          Kapp, Joshua
          de Ridder, Daniëlle
          Ryška, Aleš
          Moch, Holger
        affil: University Clermont Auvergne, INSERM U1240, Centre Jean Perrin, Clermont-Ferrand, France
      sug:
        subj:
          Carcinoma, Non-Small-Cell Lung Pathology
          Lung Neoplasms Diagnosis
          Carcinoma, Non-Small-Cell Lung
          Carcinoma, Non-Small-Cell Lung Diagnosis
          Lung Neoplasms
          Lung Neoplasms Drug Therapy
          Mutation
          Human
          Witness, Legal
          Sequence Analysis Methods
          Scales
          Ferrans and Powers Quality of Life Index
      ab: The diagnostic work-up for non-small cell lung cancer (NSCLC) requires biomarker testing to guide therapy choices. This article is the second of a two-part series. In Part 1, we summarised evidence-based recommendations for obtaining and processing small specimen samples (i.e. pre-analytical steps) from patients with advanced NSCLC. Here, in Part 2, we summarise evidence-based recommendations relating to analytical steps of biomarker testing (and associated reporting and quality assessment) of small specimen samples in NSCLC. As the number of biomarkers for actionable (genetic) targets and approved targeted therapies continues to increase, simultaneous testing of multiple actionable oncogenic drivers using next-generation sequencing (NGS) becomes imperative, as set forth in European Society for Medical Oncology guidelines. This is particularly relevant in advanced NSCLC, where tissue specimens are typically limited and NGS may help avoid tissue exhaustion compared with sequential biomarker testing. Despite guideline recommendations, significant discrepancies in access to NGS persist across Europe, primarily due to reimbursement constraints. The use of increasingly complex testing methods also has implications for the reporting of results. Molecular testing reports should include clinical interpretation with additional commentary on sample adequacy as appropriate. Molecular tumour boards are recommended to facilitate the interpretation of complex genetic information arising from NGS, and to collaboratively determine the optimal treatment for patients with NSCLC. Finally, whichever testing modality is employed, it is essential that adequate internal and external validation and quality control measures are implemented.
      pubtype: Academic Journal
      doctype:
        research
        Journal Article
      ougenre: Article
    language: English
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