Computational portraits of the tumoral microenvironment in human breast cancer.

Breast cancer is the most diagnosed cancer in humans. In recent years, myxoid and proportionated stroma have been described as clinically significant in many cancer subtypes. Here computational portraits of tumor-associated stromata were created from a machine learning (ML) classifier using QuPath t...

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Publicado en:Virchows Archiv: European Journal of Pathology Vol. 481; no. 3; pp. 367 - 386
Autores principales: Wu, Dongling, Hacking, Sean M., Chavarria, Hector, Abdelwahed, Mohammed, Nasim, Mansoor
Formato: Journal Article
Publicado: Springer Nature Sep2022
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00428-022-03376-7
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        atl: Computational portraits of the tumoral microenvironment in human breast cancer.
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          Wu, Dongling
          Hacking, Sean M.
          Chavarria, Hector
          Abdelwahed, Mohammed
          Nasim, Mansoor
        affil: Department of Pathology and Laboratory Medicine, Donald and Barbara Zucker School of Medicine at Hofstra/Northwell, Greenvale, NY, USA
      sug:
        subj:
          Breast Neoplasms Pathology
          Receptors, Cell Surface
          Female
          Cell Physiology
          Proteins
          Tyrosine
          Scales
          Female
      ab: Breast cancer is the most diagnosed cancer in humans. In recent years, myxoid and proportionated stroma have been described as clinically significant in many cancer subtypes. Here computational portraits of tumor-associated stromata were created from a machine learning (ML) classifier using QuPath to evaluate proportionated stromal area (PSA), myxoid stromal ratio (MSR), and immune stroma proportion (ISP) from whole slide images (WSI). The ML classifier was validated in independent training (n = 40) and validation (n = 109) cohorts finding MSR, PSA, and ISP to be associated with tumor stage, lymph node status, Nottingham grade, stromal differentiation (SD), tumor size, estrogen receptor (ER), progesterone receptor (PR), and receptor tyrosine-protein kinase erbB-2 (HER-2). Overall, MSR correlated better with the clinicopathologic profile than PSA and ISP. High MSR was found to be associated with high tumor stage, low ISP, and high Nottingham histologic score. As a computational biomarker, high MSR was more likely to be associated with luminal B like, Her-2 enriched, and triple-negative biomarker status when compared to luminal A like. The supervised ML superpixel approach demonstrated here can be performed by a trained pathologist to provide a faster and more uniformed approach to the analysis to the tumoral microenvironment (TME). The TME may be relevant for clinical decision-making, determining chemotherapeutic efficacy, and guiding a more overall precision-based breast cancer care.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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