Pathological processing of sentinel lymph nodes in endometrial carcinoma - routine aspects of grossing, ultra-staging, and surgico-pathological parameters in a series of 833 lymph nodes.

Sentinel lymph nodes are widely accepted in the treatment of endometrial carcinoma. Whereas surgical aspects are well studied, the pathological work-up in terms of grossing, frozen section, and the so-called ultra-staging is still a matter of debate. This results in conflicting national or center-ba...

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Publicado en:Virchows Archiv: European Journal of Pathology Vol. 481; no. 3; pp. 421 - 433
Autores principales: Rau, Tilman T., Deppeler, Mona V., Christe, Lucine, Siegenthaler, Franziska, Imboden, Sara, Papadia, Andrea, Mueller, Michael D.
Formato: Journal Article
Publicado: Springer Nature Sep2022
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s00428-022-03377-6
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        atl: Pathological processing of sentinel lymph nodes in endometrial carcinoma - routine aspects of grossing, ultra-staging, and surgico-pathological parameters in a series of 833 lymph nodes.
      aug:
        au:
          Rau, Tilman T.
          Deppeler, Mona V.
          Christe, Lucine
          Siegenthaler, Franziska
          Imboden, Sara
          Papadia, Andrea
          Mueller, Michael D.
        affil: Institute of Pathology, University Hospital Düsseldorf, Moorenstr. 5, 40235, Düsseldorf, Germany
      sug:
        subj:
          Endometrial Neoplasms Pathology
          Female
          Sentinel Lymph Node Biopsy Methods
          Lymph Nodes Pathology
          Lymph Node Excision Methods
          Proteins
          Frozen Sections
          Neoplasm Staging
          Scales
          Barthel Index
          Female
      ab: Sentinel lymph nodes are widely accepted in the treatment of endometrial carcinoma. Whereas surgical aspects are well studied, the pathological work-up in terms of grossing, frozen section, and the so-called ultra-staging is still a matter of debate. This results in conflicting national or center-based recommendations. In a series of consecutive 833 sentinel lymph nodes from 206 patients in endometrial carcinomas, we compared three different grossing techniques and the use of frozen section in terms of anatomy, detection rates, and survival. In total, 42 macro-metastases, 6 micro-metastases, and 25 nodes with isolated tumor cells were found. Lymph nodes affected at least with micro-metastasis were about 0.5cm enlarged. Detection rates in lamellation technique increased with a step of 5.9% to 8.3% in comparison to bi-valved or complete embedding. The lamellation technique presented with a slight beneficial prognosis in pN0 subgroup (OS, p=0.05), which besides size effects might be attributed to trimming loss. In frozen section, this effect was less pronounced than expected (OS, p=0.56). Ultra-staging only revealed additional micro-metastases and isolated tumor cells. Exclusively, macro-metastases showed poor survival (p<0.001). In multivariate analysis, T-stage, subtype, and lympho-vascular invasion status outperformed this staging parameter significantly. Grossing of sentinel lymph nodes is the most essential step with evidence to prefer lamellation in 2 mm steps. Step sectioning should consider widely spaced protocols to exclude macro-metastases. Frozen sections might add value to the intra-operative assessment of endometrial carcinoma in selected cases. The excellent biological behavior of cases with isolated tumor cells might question the routine application of pan-cytokeratin as ultra-staging method.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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