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Multiscale fluorescence imaging of living samples.

Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living...

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Published in:Histochemistry & Cell Biology Vol. 158; no. 4; pp. 301 - 324
Main Authors: Wu, Yicong, Shroff, Hari
Format: Journal Article
Published: Springer Nature Oct2022
Online Access:View this record in EBSCOhost
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      pub: Springer Nature
      place: New York, New York
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          Wu, Yicong
          Shroff, Hari
        affil: Laboratory of High-Resolution Optical Imaging, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 20892, Bethesda, MD, USA
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      ab: Fluorescence microscopy is a highly effective tool for interrogating biological structure and function, particularly when imaging across multiple spatiotemporal scales. Here we survey recent innovations and applications in the relatively understudied area of multiscale fluorescence imaging of living samples. We discuss fundamental challenges in live multiscale imaging and describe successful examples that highlight the power of this approach. We attempt to synthesize general strategies from these test cases, aiming to help accelerate progress in this exciting area.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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