Network Pharmacology-Integrated Molecular Docking Reveals the Expected Anticancer Mechanism of Picrorhizae Rhizoma Extract.

This study sought to explore the anticancer mechanism of Picrorhizae Rhizoma (PR) extract based on network pharmacology and molecular docking. The potential chemicals of PR were screened through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and relevant literatures. Correspo...

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Published in:BioMed Research International pp. 1 - 17
Main Authors: Hu, Xiaomeng, Zhao, Shengchao, Cai, Yi, Swain, Shasank S., Yao, Liangliang, Liu, Wei, Yan, Tingdong
Format: pictorial research tables/charts Journal Article
Published: Wiley-Blackwell 9/16/2022
Online Access:View this record in EBSCOhost
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      dt: 9/16/2022
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2022/3268773
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        atl: Network Pharmacology-Integrated Molecular Docking Reveals the Expected Anticancer Mechanism of Picrorhizae Rhizoma Extract.
      aug:
        au:
          Hu, Xiaomeng
          Zhao, Shengchao
          Cai, Yi
          Swain, Shasank S.
          Yao, Liangliang
          Liu, Wei
          Yan, Tingdong
        affil: University and College Key Lab of Natural Product Chemistry and Application in Xinjiang, School of Chemistry and Environmental Science, Yili Normal University, Yining 835000, China
      sug:
        subj:
          Network Pharmacology
          Molecular Docking Simulation
          Antineoplastic Agents Therapeutic Use
          Plant Extracts Therapeutic Use
          Medicine, Chinese Traditional
          Plant Extracts Analysis
          Human
          Molecular Structure
          Data Analysis Software
          Proteins Metabolism
          Genes
          Ontologies
          Genome
          Tumor Necrosis Factor
          Caspases
          Protein Kinases
          Epidermal Growth Factor Receptors
          Receptors, Estrogen
          Colorectal Neoplasms Drug Therapy
      ab: This study sought to explore the anticancer mechanism of Picrorhizae Rhizoma (PR) extract based on network pharmacology and molecular docking. The potential chemicals of PR were screened through the Traditional Chinese Medicine Systems Pharmacology (TCMSP) database and relevant literatures. Corresponding targets of active ingredients were found with the help of the UniProtKB database, and therapeutic targets for cancer action were screened with the help of the GeneCards database. We used Cytoscape software to construct the compound-target-pathway network of PR extract. We utilized the STRING database to obtain the protein-protein interaction (PPI) network. We used DAVID database combining Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Finally, molecular docking was employed for initial efficacy checking. We have identified 16 potential active components of PR through screening, involving 112 disease action targets. Utilizing the GeneCards database, 112 intersecting targets between PR extract and cancer were found, which mainly exerts anticancer effects by regulating tumor necrosis factor (TNF), recombinant caspase 3 (CASP3), c-Jun NH2-terminal kinase (JNK)/JUN, epidermal growth factor receptor (EGFR), and estrogen receptor-1 (ESR1) with some other target genes and pathways associated with cancer. The major anticancer species are prostate cancer, colorectal cancer, small cell lung cancer, etc. In the molecular docking study, herbactin had a strong affinity for TNF. Based on network pharmacology and molecular docking studies, PR and their compounds have demonstrated potential anticancer activities against several key targets. Our preliminary findings provide a strong foundation for further experiments with PR constituents.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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