Proteomic Analysis of Decellularized Extracellular Matrix: Achieving a Competent Biomaterial for Osteogenesis.
Decellularized ECMs have been used as biological scaffolds for tissue repair due to their tissue-specific biochemical and mechanical composition, poorly simulated by other materials. It is used as patches and powders, and it could be further processed via enzymatic digestion under acidic conditions...
| Publicado en: | BioMed Research International pp. 1 - 19 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
10/11/2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=159720890&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 159720890 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 10/11/2022 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 159720890 159720890 159720890 10.1155/2022/6884370 159720890 ppf: 1 ppct: 18 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Proteomic Analysis of Decellularized Extracellular Matrix: Achieving a Competent Biomaterial for Osteogenesis. aug: au: Monteiro-Lobato, Gabriela M. Russo, Pedro S. T. Winck, Flavia V. Catalani, Luiz H. affil: Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, São Paulo, Brazil sug: subj: Extracellular Space Cell-Matrix Junctions Biocompatible Materials Therapeutic Use Osteogenesis Drug Effects Proteomics Proteins Analysis Peptides Cell Differentiation Collagen Stem Cells Mesenchymal Stem Cells Cell Proliferation Pericardium Data Analysis Software Descriptive Statistics Tissue Engineering Organic Chemicals Digestion Peptide Hydrolases Cellular Structures Gastrointestinal System Bone Marrow ab: Decellularized ECMs have been used as biological scaffolds for tissue repair due to their tissue-specific biochemical and mechanical composition, poorly simulated by other materials. It is used as patches and powders, and it could be further processed via enzymatic digestion under acidic conditions using pepsin. However, part of the bioactivity is lost during the digestion process due to protein denaturation. Here, stepwise digestion was developed to prepare a competent biomaterial for osteogenesis from three different ECM sources. In addition, three different proteases were compared to evaluate the most effective digestion protocol for specific cellular processes. GAGs and peptide quantification showed that the stepwise method yielded a higher concentration of bioactive residues. Circular dichroism analysis also showed that the stepwise approach preserved the secondary structures better. The protein profiles of the digested ECMs were analyzed, and it was found to be highly diverse and tissue-specific. The digestion of ECM from pericardium produced peptides originated from 94 different proteins, followed by 48 proteins in ECM from tendon and 35 proteins in ECM from bone. In addition, digested products from pericardium ECM yielded increased proliferation and differentiation of bone marrow mesenchymal stem cells to mature osteoblasts. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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