Siglec-15 as a New Perspective Therapy Target in Human Giant Cell Tumor of Bone.
The main features of a giant cell tumor of bone (GCTB) are frequent recurrence and aggressive osteolysis, which leads to a poor prognosis in patients. Although the treatment methods for a GCTB, such as scraping and resection, effectively inhibit the disease, the tendency toward malignant transformat...
| Publicado en: | Current Oncology Vol. 29; no. 10; pp. 7655 - 7672 |
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| Autores principales: | , , , , , |
| Formato: | Journal Article |
| Publicado: |
MDPI
Oct2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=159900863&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 159900863 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11980052 5EKK jtl: Current Oncology issn: 11980052 maglogo: N pubinfo: dt: Oct2022 vid: 29 iid: 10 pid: 97109 pub: MDPI artinfo: ui: 159900863 10.3390/curroncol29100605 159900863 ppf: 7655 ppct: 17 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Siglec-15 as a New Perspective Therapy Target in Human Giant Cell Tumor of Bone. aug: au: Fan, Mengke Zhang, Guochuan Xie, Mingfang Liu, Xinbo Zhang, Qi Wang, Ling affil: Department of Orthopedic Research Center, Third Hospital of Hebei Medical University, Shijiazhuang 050051, China sug: ab: The main features of a giant cell tumor of bone (GCTB) are frequent recurrence and aggressive osteolysis, which leads to a poor prognosis in patients. Although the treatment methods for a GCTB, such as scraping and resection, effectively inhibit the disease, the tendency toward malignant transformation remains. Therefore, it is important to identify new treatment methods for a GCTB. In this study, we first found high Siglec-15 expression in GCTB tissues, which was significantly associated with Campanacci staging and tumor recurrence. In Spearman's analysis, Siglec-15 expression was significantly correlated with Ki-67 levels in tumor tissues. In vitro, the mRNA and protein levels of Siglec-15 were high in GCTB stromal cells (Hs737. T), and Siglec-15 knockdown inhibited the biological characteristics of GCTB stromal cells. The RNA sequencing results enabled a prediction of the downstream genes by using the Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and MCODE analyses, and the findings showed that CXCL8 was significantly regulated by Siglec-15 and might be a promising downstream target gene of Siglec-15. Therefore, Siglec-15 may be a potential immunotherapy target for a GCTB. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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