Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF-κB and MAPK Pathways in LPS-Stimulated BV-2 Microglia.
Baicalin (BA) is a major flavone from Scutellaria baicalensis Georgi and has showed significant curative effects in Parkinson's and Alzheimer's diseases. In the present study, we investigated the effects of BA on antineuroinflammation and related signaling cascade in lipopolysaccharide- (LPS-) induc...
| Published in: | BioMed Research International pp. 1 - 16 |
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| Main Authors: | , , , , , , , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
11/9/2022
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160114420&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160114420 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 11/9/2022 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 160114420 160114420 160114420 10.1155/2022/3263446 160114420 ppf: 1 ppct: 15 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Baicalin Mitigates the Neuroinflammation through the TLR4/MyD88/NF-κB and MAPK Pathways in LPS-Stimulated BV-2 Microglia. aug: au: Li, Baojing Wang, Mingming Chen, Shuai Li, Manping Zeng, Jing Wu, Saichun Tu, Yuanqing Li, Yanping Zhang, Rongping Huang, Feng Tong, Xiaoyun affil: The First Affiliated Hospital of Yunnan University of Chinese Medicine, Yunnan University of Chinese Medicine, Kunming, China sug: subj: Drugs, Chinese Herbal Pharmacodynamics Flavones Pharmacodynamics Neuroinflammation Drug Therapy Signal Transduction Drug Effects Models, Biological Brain Drugs, Chinese Herbal Pharmacokinetics Flavones Pharmacokinetics Human Inflammation Mediators Drug Effects Nitric Oxide Interleukins MicroRNA Drug Effects Gene Expression Drug Effects NF-kappa B Drug Effects Phosphorylation Drug Effects Mitogen-Activated Protein Kinases Drug Effects Molecular Docking Simulation Neurodegenerative Diseases Drug Therapy Reverse Transcriptase Polymerase Chain Reaction Blotting, Western Molecular Structure Cell Line Descriptive Statistics Data Analysis Software Analysis of Variance Post Hoc Analysis Cytokines Drug Effects Toll-Like Receptors Drug Effects ab: Baicalin (BA) is a major flavone from Scutellaria baicalensis Georgi and has showed significant curative effects in Parkinson's and Alzheimer's diseases. In the present study, we investigated the effects of BA on antineuroinflammation and related signaling cascade in lipopolysaccharide- (LPS-) induced BV-2 microglial model. The results showed that BA significantly attenuated inflammatory mediators (NO, iNOS, IL-1β, COX-2, and PGE2) and suppressed the expression of miR-155. More crucially, BA could regulate the expression of related proteins in Toll-like receptor 4 (TLR4)/myeloid differentiation protein 88 (MyD88)/nuclear factor κB (NF-κB) pathway and suppress the phosphorylation of mitogen-activated protein kinase (MAPK) family. In addition, molecular docking analysis indicated that BA binds to the amino acids Lie 63 and Tyr 65 of TLR4 by π - σ and π - π T-shaped interaction. Thus, BA suppressed the LPS-stimulated neuroinflammation in BV-2 microglia by blocking the TLR4-mediated signal transduction through TLR4/MyD88/NF-κB and MAPK pathways and inhibiting the miR-155 expression. Our findings demonstrated that BA could be a valuable therapeutic for the treatment of neuroinflammation and neurodegenerative diseases. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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