Dorsal horn volume loss and pain pathway changes in Cavalier King Charles Spaniels with syringomyelia, signs of pain, and phantom scratching.

Abstract: Central neuropathic pain is a core clinical sign of syringomyelia in humans and Cavalier King Charles Spaniel (CKCS) dogs. This histopathological study used spinal cords from CKCS dogs with syringomyelia to investigate the following conditions: (1) whether specific structural cervical spin...

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Publicado en:PAIN Vol. 163; no. 12; pp. 2365 - 2380
Autores principales: Mortensen, Danny, Thoefner, Maria Soendergaard, Agerholm, Joergen Steen, Slumstrup, Lasse, Jensen, Troels Staehelin, Bjerrum, Ole Jannik, Berendt, Mette, Nyengaard, Jens Randel
Formato: equations & formulas pictorial research tables/charts Journal Article
Publicado: Lippincott Williams & Wilkins Dec2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Dec2022
      vid: 163
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      pub: Lippincott Williams & Wilkins
      place: Baltimore, Maryland
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        10.1097/j.pain.0000000000002630
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        atl: Dorsal horn volume loss and pain pathway changes in Cavalier King Charles Spaniels with syringomyelia, signs of pain, and phantom scratching.
      aug:
        au:
          Mortensen, Danny
          Thoefner, Maria Soendergaard
          Agerholm, Joergen Steen
          Slumstrup, Lasse
          Jensen, Troels Staehelin
          Bjerrum, Ole Jannik
          Berendt, Mette
          Nyengaard, Jens Randel
        affil: Core Center for Molecular Morphology, Section for Stereology and Microscopy, Aarhus University, Aarhus, Denmark
      sug:
        subj:
          Dog Diseases
          Syringomyelia Physiopathology
          Cervical Vertebrae Analysis
          Cervical Vertebrae Pathology
          Gray Matter Pathology
          Neuralgia
          Animal Studies
          Dogs
          Histological Techniques
          Nociceptive Pain
          Glial Cells
          Magnetic Resonance Imaging
          Descriptive Statistics
          Spinal Nerve Roots
      ab: Abstract: Central neuropathic pain is a core clinical sign of syringomyelia in humans and Cavalier King Charles Spaniel (CKCS) dogs. This histopathological study used spinal cords from CKCS dogs with syringomyelia to investigate the following conditions: (1) whether specific structural cervical spinal cord entities involved in nociception were affected by loss of neuroparenchyma or other pathological changes in CKCS dogs with pain-related behaviour and phantom scratching, (2) whether pain-related behaviour or phantom scratching correlated with loss of a specific anatomical entity or upregulation of glia cells, and (3) whether syringomyelia-related lesions affected specific functional spinal cord units of nociception. Spinal cord segments C1-C8 from CKCS dogs with magnetic resonance imaging-confirmed syringomyelia and clinical signs of pain and phantom scratching (n = 8) were compared with those from CKCS dogs without syringomyelia (n = 4). Dogs with unilateral scratching (n = 7) had a volume loss ( P = 0.043) of the dorsal horn laminae I-III in the ipsilateral side compared with the contralateral dorsal horn. A clear pattern of ipsilateral changes in the dorsal root entry zone characterised by deafferentation and reorganization of first-order axons into deeper laminae was found in cases with lateralised scratching. Significant changes in cell number density were not found for astrocytes or microglia, suggesting that the dogs represented cases of end-stage syringomyelia and thus could not reveal astrogliosis and microgliosis, which may be involved in the early phases of syrinx development and phantom scratching. The present relationship between clinical findings and dorsal horn and pain pathway pathology in CKCS dogs suggests that these dogs may be of interest as a supplement to experimental model pain research.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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