Chromatin accessibility analysis reveals distinct functions for HDAC and EZH2 activities in early appendage regeneration.
Xenopus tropicalis tadpoles have the capacity for scarless regeneration of appendages including the limb and tail. Following injury, transcriptional programs must be activated and inactivated with high spatial and temporal resolution to result in a properly patterned appendage. Functional studies ha...
| Publicado en: | Wound Repair & Regeneration Vol. 30; no. 6; pp. 707 - 726 |
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| Autores principales: | , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Nov2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160352287&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160352287 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Nov2022 vid: 30 iid: 6 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 160352287 160352287 160352287 10.1111/wrr.13057 160352287 ppf: 707 ppct: 19 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Chromatin accessibility analysis reveals distinct functions for HDAC and EZH2 activities in early appendage regeneration. aug: au: Arbach, Hannah E. Harland‐Dunaway, Marcus Braden, Christopher Chitsazan, Alexander D. Pickering, Eleanor Patel, Jeet H. Wills, Andrea E. affil: Department of Biochemistry, University of Washington, Seattle Washington,, USA sug: subj: Sex Chromatin Epigenomics Larva Body Organs Regeneration Wound Healing Animal Studies Animals Genomics Blotting, Western Fluorescent Antibody Technique Experimental Studies Funding Source Gene Expression ab: Xenopus tropicalis tadpoles have the capacity for scarless regeneration of appendages including the limb and tail. Following injury, transcriptional programs must be activated and inactivated with high spatial and temporal resolution to result in a properly patterned appendage. Functional studies have established that histone‐modifying enzymes that act to close chromatin are required for regeneration, but the genomic regions sensitive to these activities are not fully established. Here we show that early inhibition of HDAC or EZH2 activity results in incomplete tail regeneration. To identify how each of these perturbations impacts chromatin accessibility, we applied an assay for transposase‐accessible chromatin (ATAC‐seq) to HDAC or EZH2‐inhibited regenerating tadpoles. We find that neither perturbation results in a global increase in chromatin accessibility, but that both inhibitors have targeted effects on chromatin accessibility and gene expression. Upon HDAC inhibition, regulatory regions neighbouring genes associated with neuronal regeneration are preferentially accessible, whereas regions associated with immune response and apoptosis are preferentially accessible following EZH2 inhibition. Together, these results suggest distinct roles for these two chromatin‐closing activities in appendage regeneration. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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