Comparison of Acoustofluidic and Static Systems for Ultrasound-Mediated Molecular Delivery to T Lymphocytes.
Continuous-flow acoustofluidic technologies can potentially improve processing of T lymphocytes for cell therapies by addressing the limitations with viral and non-viral delivery methods. The objective of this study was to assess the intracellular delivery efficiency with acoustofluidic treatment co...
| Published in: | Ultrasound in Medicine & Biology Vol. 49; no. 1; pp. 90 - 106 |
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| Main Authors: | , , , , , |
| Format: | research Journal Article |
| Published: |
Elsevier B.V.
Jan2023
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160398968&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160398968 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03015629 JJ6 jtl: Ultrasound in Medicine & Biology issn: 03015629 maglogo: N pubinfo: dt: Jan2023 vid: 49 iid: 1 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 160398968 160398968 NLM36241589 160398968 10.1016/j.ultrasmedbio.2022.08.005 NLM36241589 160398968 ppf: 90 ppct: 16 formats: tig: atl: Comparison of Acoustofluidic and Static Systems for Ultrasound-Mediated Molecular Delivery to T Lymphocytes. aug: au: Centner, Connor S. Moore, John T. Baxter, Mary E. Yaddanapudi, Kavitha Bates, Paula J. Kopechek, Jonathan A. affil: Department of Bioengineering, University of Louisville, Louisville, Kentucky, USA sug: subj: Equipment and Supplies T Lymphocytes Drug Delivery Systems Methods Ultrasonography Genetic Techniques ab: Continuous-flow acoustofluidic technologies can potentially improve processing of T lymphocytes for cell therapies by addressing the limitations with viral and non-viral delivery methods. The objective of this study was to assess the intracellular delivery efficiency with acoustofluidic treatment compared with that of static ultrasound treatment. Optimization of parameters in acoustofluidic and static configurations was performed by assessing intracellular delivery of a fluorescent compound (calcein) in viable human Jurkat T lymphocytes. Ultrasound pressure and the concentration of cationic phospholipid-coated microbubbles influenced calcein delivery in both systems. In the static system, a treatment time of 45 s increased molecular delivery compared with 0-30 s (p < 0.01). Refined parameters were used to assess molecular delivery of small and large compounds (0.6-kDa calcein and 150-kDa fluorescein isothiocyanate-dextran, respectively) after ultrasound treatment with the acoustofluidic or static systems. Molecular delivery was similar with refined parameters for acoustofluidic treatment and static treatment (p > 0.05), even though acoustofluidic treatment had lower microbubble concentration (24 μg/mL vs. 94 μg/mL) and shorter treatment time (∼2-3 s vs. 45 s). This study indicates that the acoustofluidic system can significantly enhance intracellular molecular delivery, which could potentially enable acoustofluidic cell transfection during continuous flow processing for manufacture of cell therapies or other applications. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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