Humoral immunity in dually vaccinated SARS-CoV-2-naïve individuals and in booster-vaccinated COVID-19-convalescent subjects.

Background: The immune response to COVID-19-vaccination differs between naïve vaccinees and those who were previously infected with SARS-CoV-2. Longitudinal quantitative and qualitative serological differences in these two distinct immunological subgroups in response to vaccination are currently not...

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Publicado en:Infection Vol. 50; no. 6; pp. 1475 - 1482
Autores principales: Glück, Vivian, Tydykov, Leonid, Mader, Anna-Lena, Warda, Anne-Sophie, Bertok, Manuela, Weidlich, Tanja, Gottwald, Christine, Köstler, Josef, Salzberger, Bernd, Wagner, Ralf, Koller, Michael, Gessner, André, Schmidt, Barbara, Glück, Thomas, Peterhoff, David
Formato: research tables/charts Journal Article
Publicado: Springer Nature Dec2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Dec2022
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      pub: Springer Nature
      place: New York, New York
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        10.1007/s15010-022-01817-8
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        atl: Humoral immunity in dually vaccinated SARS-CoV-2-naïve individuals and in booster-vaccinated COVID-19-convalescent subjects.
      aug:
        au:
          Glück, Vivian
          Tydykov, Leonid
          Mader, Anna-Lena
          Warda, Anne-Sophie
          Bertok, Manuela
          Weidlich, Tanja
          Gottwald, Christine
          Köstler, Josef
          Salzberger, Bernd
          Wagner, Ralf
          Koller, Michael
          Gessner, André
          Schmidt, Barbara
          Glück, Thomas
          Peterhoff, David
        affil: Institute for Clinical Microbiology and Hygiene, University Hospital Regensburg, Regensburg, Germany
      sug:
        subj:
          COVID-19 Vaccines
          COVID-19 Drug Therapy
          Immunity
          Antibody Formation
          Immunization, Secondary
          Human
          Immunization
          Quantitative Studies
          Qualitative Studies
          Enzyme-Linked Immunosorbent Assay
          Neutralization Tests
          Immunoglobulins
          Antigen-Antibody Reactions
      ab: Background: The immune response to COVID-19-vaccination differs between naïve vaccinees and those who were previously infected with SARS-CoV-2. Longitudinal quantitative and qualitative serological differences in these two distinct immunological subgroups in response to vaccination are currently not well studied. Methods: We investigate a cohort of SARS-CoV-2-naïve and COVID-19-convalescent individuals immediately after vaccination and 6 months later. We use different enzyme-linked immunosorbent assay (ELISA) variants and a surrogate virus neutralization test (sVNT) to measure IgG serum titers, IgA serum reactivity, IgG serum avidity and neutralization capacity by ACE2 receptor competition. Results: Anti-receptor-binding domain (RBD) antibody titers decline over time in dually vaccinated COVID-19 naïves whereas titers in single dose vaccinated COVID-19 convalescents are higher and more durable. Similarly, antibody avidity is considerably higher among boosted COVID-19 convalescent subjects as compared to dually vaccinated COVID-19-naïve subjects. Furthermore, sera from boosted convalescents inhibited the binding of spike-protein to ACE2 more efficiently than sera from dually vaccinated COVID-19-naïve subjects. Conclusions: Long-term humoral immunity differs substantially between dually vaccinated SARS-CoV-2-naïve and COVID-19-convalescent individuals. Booster vaccination after COVID-19 induces a more durable humoral immune response in terms of magnitude and quality as compared to two-dose vaccination in a SARS-CoV-2-naïve background.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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