Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array.
Background: Surgical site infection (SSI) is a post-operative complication of high concern with adverse impact on patient prognosis and public health systems. Recently, SSI pathogens have experienced a change in microbial profile with increasing reports of non-tuberculous mycobacteria (NTM) as impor...
| Publicado en: | Infection Vol. 50; no. 6; pp. 1557 - 1564 |
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| Autores principales: | , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Springer Nature
Dec2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160459273&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160459273 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03008126 NXO jtl: Infection issn: 03008126 maglogo: N pubinfo: dt: Dec2022 vid: 50 iid: 6 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 160459273 157487002 160459273 160459273 10.1007/s15010-022-01864-1 160459273 ppf: 1557 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Surgical site infections by atypical mycobacteria: prevalence and species characterization using MALDI-TOF and molecular LCD chip array. aug: au: Gad, Maha A. Khairat, Sahar M. Salama, Amira M. A. Abd Elmoez, Omnia A. Soliman, Noha S. affil: Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt sug: subj: Surgical Wound Infection Epidemiology Mycobacterium Infections Epidemiology Surgical Wound Infection Microbiology Microarray Analysis Methods Biochips Mass Spectrometry Methods Polymerase Chain Reaction Methods Epidemiology, Molecular Human Molecular Biology Microbial Culture and Sensitivity Tests Chromatography, Liquid Methods Descriptive Statistics Prevalence Surgical Wound Infection Diagnosis Mycobacterium Infections Diagnosis Mycobacterium Infections Drug Therapy Surgical Wound Infection Drug Therapy Technology ab: Background: Surgical site infection (SSI) is a post-operative complication of high concern with adverse impact on patient prognosis and public health systems. Recently, SSI pathogens have experienced a change in microbial profile with increasing reports of non-tuberculous mycobacteria (NTM) as important pathogens. Aim: of the study The study aimed to detect the prevalence of NTM among cases with SSIs and describe their species using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) and PCR-based microarray. Methods: The study was conducted with 192 pus samples collected from patients with SSI. Mycobacterial investigations were done in the form of Ziehl–Neelsen (ZN) smears for acid-fast bacilli, automated mycobacterial culture to isolate mycobacteria, followed by immunochromatography test to predict NTM. NTM-positive cultures were tested by MALDI -TOF MS and PCR-based microarray to reach species-level identification. Results: Mycobacterial growth was found in 11/192 samples (5.7%) and identified as 4 NTM and 7 M. tuberculosis isolates with prevalence of 2.1% and 3.64%, respectively. The NTM species were described by MALDI-TOF as M. abscessus, M. porcinum, M. bacteremicum, and M. gordonae. Microarray agreed with MALDI-TOF in identifying one isolate (M. abscessus), while two isolates were classified as belonging to broad groups and one isolate failed to be identified. Conclusions: The prevalence of NTM among SSI was found to be low, yet have to be considered in the diagnosis of mycobacteria. Employing advanced technologies in diagnosis is recommended to guide for appropriate treatment. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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