CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data.
Background: Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced pluripotent stem cells (hiPSCs), are lacking. E...
| Publicado en: | BMC Neuroscience Vol. 23; no. 1; pp. 1 - 15 |
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| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts tracings Journal Article |
| Publicado: |
BioMed Central
11/30/2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160504058&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160504058 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14712202 1CI8 jtl: BMC Neuroscience issn: 14712202 maglogo: N pubinfo: dt: 11/30/2022 vid: 23 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 160504058 160504058 NLM36451089 160504058 10.1186/s12868-022-00751-7 NLM36451089 160504058 ppf: 1 ppct: 14 formats: tig: atl: CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data. aug: au: Tippani, Madhavi Pattie, Elizabeth A. Davis, Brittany A. Nguyen, Claudia V. Wang, Yanhong Sripathy, Srinidhi Rao Maher, Brady J. Martinowich, Keri Jaffe, Andrew E. Page, Stephanie Cerceo affil: Lieber Institute for Brain Development, Johns Hopkins Medical Campus, 855 North Wolfe Street, Suite 300, 21205, Baltimore, MD, USA sug: subj: Calcium Stem Cells Cell Line Neurons Image Processing, Computer Assisted Animal Studies Rats ab: Background: Calcium imaging is a powerful technique for recording cellular activity across large populations of neurons. However, analysis methods capable of single-cell resolution in cultured neurons, especially for cultures derived from human induced pluripotent stem cells (hiPSCs), are lacking. Existing methods lack scalability to accommodate high-throughput comparisons between multiple lines, across developmental timepoints, or across pharmacological manipulations.Results: To address this need we developed CaPTure, a scalable, automated Ca2+ imaging analysis pipeline ( https://github.com/LieberInstitute/CaPTure ). CaPTuredetects neurons, classifies and quantifies spontaneous activity, quantifies synchrony metrics, and generates cell- and network-specific metrics that facilitate phenotypic discovery. The method is compatible with parallel processing on computing clusters without requiring significant user input or parameter modification.Conclusion: CaPTure allows for rapid assessment of neuronal activity in cultured cells at cellular resolution, rendering it amenable to high-throughput screening and phenotypic discovery. The platform can be applied to both human- and rodent-derived neurons and is compatible with many imaging systems. pubtype: Academic Journal doctype: pictorial research tables/charts tracings Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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