UPLC-MS/MS and GC-MS based metabolites profiling of Moringa oleifera seed with its anti- Helicobacter pylori and anti-inflammatory activities.
Compared to its leaf, few studies have been reported on the seeds of Moringa oleifera Lam. Metabolites profiling analysis of M. oleifera seed methanolic extract (ME) and its fixed oil (MO) was attempted via LC/MS and GC/MS. LC/MS analysis of M. oleifera seeds annotated 84 peaks of which glucosinolat...
| Publicado en: | Natural Product Research Vol. 36; no. 24; pp. 6433 - 6439 |
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| Autores principales: | , , , |
| Formato: | Journal Article |
| Publicado: |
Taylor & Francis Ltd
Dec2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160588698&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160588698 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14786419 RFT jtl: Natural Product Research issn: 14786419 maglogo: Y pubinfo: dt: Dec2022 vid: 36 iid: 24 pid: 377 pub: Taylor & Francis Ltd place: Philadelphia, Pennsylvania artinfo: ui: 160588698 155112933 10.1080/14786419.2022.2037088 160588698 ppf: 6433 ppct: 6 formats: tig: atl: UPLC-MS/MS and GC-MS based metabolites profiling of Moringa oleifera seed with its anti- Helicobacter pylori and anti-inflammatory activities. aug: au: Sayed, Abeer M. El Omar, Farghaly A. Emam, Marwa Mostafa Abdel-Aziz Farag, Mohamed A. affil: Pharmacognosy Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt sug: ab: Compared to its leaf, few studies have been reported on the seeds of Moringa oleifera Lam. Metabolites profiling analysis of M. oleifera seed methanolic extract (ME) and its fixed oil (MO) was attempted via LC/MS and GC/MS. LC/MS analysis of M. oleifera seeds annotated 84 peaks of which glucosinolates and their corresponding acetyl isomers were abundant. GC/MS of seed oil revealed the abundance of fatty acids with oleic acid at 34.3%. ME exhibited significant anti-Helicobacter pylori activity with MIC50 0.92 µg/mL, nearly one-half that of Clarithromycin. Fixed oil (MO) showed a nonselective anti-inflammatory effect with IC50= 24.4 ± 0.8 µg/mL correlated to Ibuprofen. To unravel the mechanism of the anti-H. pylori activity a molecular docking study of the principal components of the ME has been performed, using H. pylori urease enzyme. Interactions with Ni2+ ions and amino acid residue in the active site, which are crucial for the enzyme's biochemical role, are evidenced. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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