Altered Gastric Microbiota and Inflammatory Cytokine Responses in Patients with Helicobacter pylori -Negative Gastric Cancer.
The role of the gastric mucosal microbiome in Helicobacter pylori-negative gastric cancer (GC) remains unclear. Therefore, we aimed to characterize the microbial alterations and host inflammatory cytokine responses in H. pylori-negative GC. Gastric mucosal samples were obtained from 137 H. pylori-ne...
| Publicado en: | Nutrients Vol. 14; no. 23; pp. 4981 - 4996 |
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| Autores principales: | , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
MDPI
Dec2022
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160738134&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160738134 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 20726643 B0TT jtl: Nutrients issn: 20726643 maglogo: N pubinfo: dt: Dec2022 vid: 14 iid: 23 pid: 97109 pub: MDPI artinfo: ui: 160738134 160738134 160738134 10.3390/nu14234981 160738134 ppf: 4981 ppct: 15 formats: tig: atl: Altered Gastric Microbiota and Inflammatory Cytokine Responses in Patients with Helicobacter pylori -Negative Gastric Cancer. aug: au: Kim, Han-Na Kim, Min-Jeong Jacobs, Jonathan P. Yang, Hyo-Joon affil: Medical Research Institute, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul 03181, Republic of Korea sug: subj: Gastric Mucosa Microbiology Cytokines Analysis Inflammation Stomach Neoplasms Human Cancer Patients Gastritis Metaplasia Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Gene Expression RNA, Messenger Analysis Tumor Necrosis Factor Analysis Interleukins Analysis Transforming Growth Factor beta Analysis Haemophilus Campylobacter ab: The role of the gastric mucosal microbiome in Helicobacter pylori-negative gastric cancer (GC) remains unclear. Therefore, we aimed to characterize the microbial alterations and host inflammatory cytokine responses in H. pylori-negative GC. Gastric mucosal samples were obtained from 137 H. pylori-negative patients with GC (n = 45) and controls (chronic gastritis or intestinal metaplasia, n = 92). We performed 16S rRNA gene sequencing (n = 67), a quantitative reverse transcription-polymerase chain reaction to determine the relative mRNA expression levels of TNF (tumor necrosis factor), IL1B (interleukin 1 beta), IL6 (interleukin 6), CXCL8 (C-X-C motif chemokine ligand 8), IL10 (interleukin 10), IL17A (interleukin 17A), TGFB1 (transforming growth factor beta 1) (n = 113), and the correlation analysis between sequencing and expression data (n = 47). Gastric mucosal microbiota in patients with GC showed reduced diversity and a significantly different composition compared to that of the controls. Lacticaseibacillus was significantly enriched, while Haemophilus and Campylobacter were depleted in the cancer group compared to the control group. These taxa could distinguish the two groups in a random forest algorithm. Moreover, the combined relative abundance of these taxa, a GC microbiome index, significantly correlated with gastric mucosal IL1B expression, which was elevated in the cancer group. Overall, altered gastric mucosal microbiota was found to be associated with increased mucosal IL1B expression in H. pylori-negative GC. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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