Short-Term In Vitro ROS Detection and Oxidative Stress Regulators in Epiretinal Membranes and Vitreous from Idiopathic Vitreoretinal Diseases.
Background. A plethora of inflammatory, angiogenic, and tissue remodeling factors has been reported in idiopathic epiretinal membranes (ERMs). Herein we focused on the expression of a few mediators (oxidative, inflammatory, and angiogenic/vascular factors) by means of short-term vitreal cell culture...
| Publicado en: | BioMed Research International pp. 1 - 13 |
|---|---|
| Autores principales: | , , , , , , , |
| Formato: | diagnostic images pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
12/16/2022
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=160843124&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 160843124 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 12/16/2022 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 160843124 160843124 160843124 10.1155/2022/7497816 160843124 ppf: 1 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Short-Term In Vitro ROS Detection and Oxidative Stress Regulators in Epiretinal Membranes and Vitreous from Idiopathic Vitreoretinal Diseases. aug: au: Balzamino, Bijorn Omar Dinice, Lucia Cacciamani, Andrea Re, Agnese Scarinci, Fabio Bruno, Luca Cosimi, Pamela Micera, Alessandra affil: Research Laboratories in Ophthalmology, IRCCS-Fondazione Bietti, Rome, Italy sug: subj: Reactive Oxygen Species Analysis Oxidative Stress Evaluation Vitreous Body Metabolism Retinal Diseases Pathology Biological Markers Metabolism Inflammation Mediators Metabolism Cell Line Metabolism Molecular Biology Human In Vitro Studies Retinal Diseases Male Female Aged Cell Culture Techniques Eye Surgery Nitric Oxide Synthases Metabolism Vascular Endothelial Growth Factors Metabolism Interferons Metabolism Interleukins Metabolism Enzyme-Linked Immunosorbent Assay Blotting, Western NF-kappa B Metabolism Intercellular Signaling Peptides and Proteins Metabolism Carrier Proteins Metabolism NF-E2-Related Factor 2 Metabolism Nitric Oxide Metabolism Polymerase Chain Reaction Severity of Illness Aged: 65+ years Male Female ab: Background. A plethora of inflammatory, angiogenic, and tissue remodeling factors has been reported in idiopathic epiretinal membranes (ERMs). Herein we focused on the expression of a few mediators (oxidative, inflammatory, and angiogenic/vascular factors) by means of short-term vitreal cell cultures and biomolecular analysis. Methods. Thirty-nine (39) ERMs and vitreal samples were collected at the time of vitreoretinal surgery and biomolecular analyses were performed in clear vitreous, vitreal cell pellets, and ERMs. ROS products and iNOS were investigated in adherent vitreal cells and/or ERMs, and iNOS, VEGF, Ang-2, IFNγ, IL18, and IL22 were quantified in vitreous (ELISA/Ella, IF/WB); transcripts specific for iNOS, p65NFkB, KEAP1, NRF2, and NOX1/NOX4 were detected in ERMs (PCR). Biomolecular changes were analyzed and correlated with disease severity. Results. The higher ROS production was observed in vitreal cells at stage 4, and iNOS was found in ERMs and increased in the vitreous as early as at stage 3. Both iNOS and NOX4 were upregulated at all stages, while p65NFkB was increased at stage 3. iNOS and NOX1 were positively and inversely related with p65NFkB. While NOX4 transcripts were always upregulated, NRF2 was upregulated at stage 3 and inverted at stage 4. No significant changes occurred in the release of angiogenic (VEGF, Ang-2) and proinflammatory (IL18, IL22 and IFNγ) mediators between all stages investigated. Conclusions. ROS production was strictly associated with iNOS and NOX4 overexpression and increased depending on ERM stadiation. The higher iNOS expression occurred as early as stage 3, with respect to p65NFkB and NRF2. These last mediators might have potential prognostic values in ERMs as representative of an underneath retinal damage. pubtype: Academic Journal doctype: diagnostic images pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|