Multimodal Lung Cancer Subtyping Using Deep Learning Neural Networks on Whole Slide Tissue Images and MALDI MSI.

Simple Summary: For the effective treatment of lung cancer patients, correct tumor subtyping is of utmost importance, but it is often challenging in clinical routine. Using artificial intelligence and combining information from digital microscopy and matrix-assisted laser desorption/ionization mass...

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Publicado en:Cancers Vol. 14; no. 24; pp. 6181 - 6202
Autores principales: Janßen, Charlotte, Boskamp, Tobias, Le'Clerc Arrastia, Jean, Otero Baguer, Daniel, Hauberg-Lotte, Lena, Kriegsmann, Mark, Kriegsmann, Katharina, Steinbuß, Georg, Casadonte, Rita, Kriegsmann, Jörg, Maaß, Peter
Formato: pictorial research tables/charts Journal Article
Publicado: MDPI 12/15/2022
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 12/15/2022
      vid: 14
      iid: 24
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      pub: MDPI
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        160958762
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        10.3390/cancers14246181
        160958762
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        atl: Multimodal Lung Cancer Subtyping Using Deep Learning Neural Networks on Whole Slide Tissue Images and MALDI MSI.
      aug:
        au:
          Janßen, Charlotte
          Boskamp, Tobias
          Le'Clerc Arrastia, Jean
          Otero Baguer, Daniel
          Hauberg-Lotte, Lena
          Kriegsmann, Mark
          Kriegsmann, Katharina
          Steinbuß, Georg
          Casadonte, Rita
          Kriegsmann, Jörg
          Maaß, Peter
        affil: Center for Industrial Mathematics (ZeTeM), University of Bremen, 28359 Bremen, Germany
      sug:
        subj:
          Carcinoma, Non-Small-Cell Lung Diagnosis
          Carcinoma, Non-Small-Cell Lung Classification
          Deep Learning
          Neural Networks (Computer)
          Mass Spectrometry Methods
          Microscopy, Virtual
          Diagnosis, Computer Assisted
          Human
          Algorithms
          Adenocarcinoma of Lung Diagnosis
          Carcinoma, Squamous Cell Diagnosis
          Staining and Labeling
          Descriptive Statistics
      ab: Simple Summary: For the effective treatment of lung cancer patients, correct tumor subtyping is of utmost importance, but it is often challenging in clinical routine. Using artificial intelligence and combining information from digital microscopy and matrix-assisted laser desorption/ionization mass spectrometry imaging data have the potential to support the pathologist's decision-making process. We present a classification algorithm to distinguish between adenocarcinoma and squamous cell carcinoma of the lung based on the automatic detection of tumor areas in whole tissue sections and the determination of the tumor subtype with high accuracy. Artificial intelligence (AI) has shown potential for facilitating the detection and classification of tumors. In patients with non-small cell lung cancer, distinguishing between the most common subtypes, adenocarcinoma (ADC) and squamous cell carcinoma (SqCC), is crucial for the development of an effective treatment plan. This task, however, may still present challenges in clinical routine. We propose a two-modality, AI-based classification algorithm to detect and subtype tumor areas, which combines information from matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) data and digital microscopy whole slide images (WSIs) of lung tissue sections. The method consists of first detecting areas with high tumor cell content by performing a segmentation of the hematoxylin and eosin-stained (H&E-stained) WSIs, and subsequently classifying the tumor areas based on the corresponding MALDI MSI data. We trained the algorithm on six tissue microarrays (TMAs) with tumor samples from N = 232 patients and used 14 additional whole sections for validation and model selection. Classification accuracy was evaluated on a test dataset with another 16 whole sections. The algorithm accurately detected and classified tumor areas, yielding a test accuracy of 94.7% on spectrum level, and correctly classified 15 of 16 test sections. When an additional quality control criterion was introduced, a 100% test accuracy was achieved on sections that passed the quality control (14 of 16). The presented method provides a step further towards the inclusion of AI and MALDI MSI data into clinical routine and has the potential to reduce the pathologist's work load. A careful analysis of the results revealed specific challenges to be considered when training neural networks on data from lung cancer tissue.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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