Identification of Methylation Signatures and Rules for Sarcoma Subtypes by Machine Learning Methods.

Sarcoma, the second common type of solid tumor in children and adolescents, has a wide variety of subtypes that are often not properly diagnosed at an early stage, leading to late metastases and causing serious loss of life and property to patients and families. It exhibits a high degree of heteroge...

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Publicado en:BioMed Research International pp. 1 - 12
Autores principales: Ren, Jingxin, Zhou, XianChao, Guo, Wei, Feng, KaiYan, Huang, Tao, Cai, Yu-Dong
Formato: equations & formulas research tables/charts Journal Article
Publicado: Wiley-Blackwell 12/28/2022
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: BioMed Research International
      issn: 23146133
      maglogo: N
    pubinfo:
      dt: 12/28/2022
      pid: 480
      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2022/5297235
        161035707
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      ppct: 11
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              type: T
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              type: P
      tig:
        atl: Identification of Methylation Signatures and Rules for Sarcoma Subtypes by Machine Learning Methods.
      aug:
        au:
          Ren, Jingxin
          Zhou, XianChao
          Guo, Wei
          Feng, KaiYan
          Huang, Tao
          Cai, Yu-Dong
        affil: School of Life Sciences, Shanghai University, Shanghai 200444, China
      sug:
        subj:
          Machine Learning Methods
          Sarcoma Classification
          DNA Methylation
          Early Diagnosis
          Sarcoma Diagnosis
          Biological Markers
          Algorithms
          Decision Trees
          Random Forest
          Models, Theoretical
          Gene Expression
          Epigenomics
          Human
      ab: Sarcoma, the second common type of solid tumor in children and adolescents, has a wide variety of subtypes that are often not properly diagnosed at an early stage, leading to late metastases and causing serious loss of life and property to patients and families. It exhibits a high degree of heterogeneity at the cellular, molecular, and epigenetic levels, where DNA methylation has been proposed to play a role in the diagnosis of sarcoma subtypes. Thus, this study is aimed at finding potential biomarkers at the DNA methylation level to distinguish different sarcoma subtypes. A machine learning process was designed to analyse sarcoma samples, each of which was represented by lots of methylation sites. Irrelevant sites were removed using the Boruta method, and remaining sites related to the target variables were kept for further analyses. Afterward, three feature ranking methods (LASSO, LightGBM, and MCFS) were adopted to rank these features, and six classification models were constructed by combining incremental feature selection and two classification algorithms (decision tree and random forest). Among these models, the performance of RF model was higher than that of DT model under all three ranking conditions. The specific expression of genes obtained from the annotation of highly correlated methylation site features, such as PRKAR1B, INPP5A, and GLI3, was proven to be associated with sarcoma by publications. Moreover, the quantitative rules obtained by decision tree algorithm helped us to understand the essential differences between various sarcoma types and classify sarcoma subtypes, providing a new means of clinical identification and determining new therapeutic targets.
      pubtype: Academic Journal
      doctype:
        equations & formulas
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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