Machine Learning Analysis of the Cerebrovascular Thrombi Lipidome in Acute Ischemic Stroke.

OBJECTIVE: The aim of this study was to identify a signature lipid profile from cerebral thrombi in acute ischemic stroke (AIS) patients at the time of ictus. METHODS: We performed untargeted lipidomics analysis using liquid chromatography-mass spectrometry on cerebral thrombi taken from a nonprobab...

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Publicado en:Journal of Neuroscience Nursing Vol. 55; no. 1; pp. 10 - 18
Autores principales: Martha, Sarah R., Levy, Samuel H., Federico, Emma, Levitt, Michael R., Walker, Melanie
Formato: research tables/charts Journal Article
Publicado: Lippincott Williams & Wilkins Feb2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: Feb2023
      vid: 55
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      pub: Lippincott Williams & Wilkins
      place: Baltimore, Maryland
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        161203668
        161203668
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        10.1097/JNN.0000000000000682
        161203668
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        atl: Machine Learning Analysis of the Cerebrovascular Thrombi Lipidome in Acute Ischemic Stroke.
      aug:
        au:
          Martha, Sarah R.
          Levy, Samuel H.
          Federico, Emma
          Levitt, Michael R.
          Walker, Melanie
        affil: Samuel H. Levy, Department of Neurological Surgery and Stroke and Applied Neuroscience (SANS) Center, University of Washington, Seattle, WA.
      sug:
        subj:
          Machine Learning
          Ischemic Stroke
          Intracranial Thrombosis
          Metabolomics
          Human
          Chromatography, Liquid
          Mass Spectrometry
          Nonprobability Sample
          Convenience Sample
          Random Forest
          Biological Markers
          Research, Nursing
      ab: OBJECTIVE: The aim of this study was to identify a signature lipid profile from cerebral thrombi in acute ischemic stroke (AIS) patients at the time of ictus. METHODS: We performed untargeted lipidomics analysis using liquid chromatography-mass spectrometry on cerebral thrombi taken from a nonprobability, convenience sampling of adult subjects (≥18 years old, n = 5) who underwent thrombectomy for acute cerebrovascular occlusion. The data were classified using random forest, a machine learning algorithm. RESULTS: The top 10 metabolites identified from the random forest analysis were of the glycerophospholipid species and fatty acids. CONCLUSION: Preliminary analysis demonstrates feasibility of identification of lipid metabolomic profiling in cerebral thrombi retrieved from AIS patients. Recent advances in omic methodologies enable lipidomic profiling, which may provide insight into the cellular metabolic pathophysiology caused by AIS. Understanding of lipidomic changes in AIS may illuminate specific metabolite and lipid pathways involved and further the potential to develop personalized preventive strategies.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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