Topical vanadate improves tensile strength and alters collagen organisation of excisional wounds in a mouse model.
Wound dehiscence, oftentimes a result of the poor tensile strength of early healing wounds, is a significant threat to the post‐operative patient, potentially causing life‐threatening complications. Vanadate, a protein tyrosine phosphatase inhibitor, has been shown to alter the organisation of depos...
| Publicado en: | Wound Repair & Regeneration Vol. 31; no. 1; pp. 77 - 87 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jan/Feb2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=161473482&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 161473482 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jan/Feb2023 vid: 31 iid: 1 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 161473482 160751507 161473482 161473482 10.1111/wrr.13062 161473482 ppf: 77 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Topical vanadate improves tensile strength and alters collagen organisation of excisional wounds in a mouse model. aug: au: Lintel, Hendrik Abbas, Darren B. Mackay, Duncan J. Griffin, Michelle Lavin, Christopher V. Berry, Charlotte E. Guardino, Nicholas J. Guo, Jason L. Momeni, Arash Mackay, Donald R. Longaker, Michael T. Wan, Derrick C. affil: Hagey Laboratory for Pediatric Regenerative Medicine, Stanford University School of Medicine, Stanford California,, USA sug: subj: Vanadates Pharmacodynamics Vanadates Therapeutic Use Vanadates Administration and Dosage Wound Healing Tensile Strength Drug Effects Collagen Drug Effects Surgical Wound Prevention and Control Animal Studies Mice Models, Biological Skin Injuries Vanadates Metabolism Biomechanics Surgical Wound Dehiscence Prevention and Control ab: Wound dehiscence, oftentimes a result of the poor tensile strength of early healing wounds, is a significant threat to the post‐operative patient, potentially causing life‐threatening complications. Vanadate, a protein tyrosine phosphatase inhibitor, has been shown to alter the organisation of deposited collagen in healing wounds and significantly improve the tensile strength of incisional wounds in rats. In this study, we sought to explore the effects of locally administered vanadate on tensile strength and collagen organisation in both the early and remodelling phases of excisional wound healing in a murine model. Wild‐type mice underwent stented excisional wounding on their dorsal skin and were divided equally into three treatment conditions: vanadate injection, saline injection control and an untreated control. Tensile strength testing, in vivo suction Cutometer analysis, gross wound measurements and histologic analysis were performed during healing, immediately upon wound closure, and after 4 weeks of remodelling. We found that vanadate treatment significantly increased the tensile strength of wounds and their stiffness relative to control wounds, both immediately upon healing and into the remodelling phase. Histologic analysis revealed that these biomechanical changes were likely the result of increased collagen deposition and an altered collagen organisation composed of thicker and distinctly organised collagen bundles. Given the risk that dehiscence poses to all operative patients, vanadate presents an interesting therapeutic avenue to improve the strength of post‐operative wounds and unstable chronic wounds to reduce the risk of dehiscence. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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