Comparison of Inflation and Ventilation with Hydrogen Sulfide during the Warm Ischemia Phase on Ischemia-Reperfusion Injury in a Rat Model of Non-Heart-Beating Donor Lung Transplantation.

Donor lung ventilation and inflation during the warm ischemia could attenuate ischemia-reperfusion injury (IRI) after lung transplantation. Hydrogen sulfide (H2S), as a kind of protective gas, has demonstrated the antilung IRI effect. This study is aimed at observing the different methods of adminis...

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Publicado en:BioMed Research International Vol. 2023; pp. 1 - 11
Autores principales: Yan, Junwei, Zhang, Bing, Yu, Junmin, Wang, Xuan, Zhao, Han, Li, Guangqi, Yin, Yanwei, Meng, Chao
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell 2/2/2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2/2/2023
      vid: 2023
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        161719530
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        10.1155/2023/3645304
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        atl: Comparison of Inflation and Ventilation with Hydrogen Sulfide during the Warm Ischemia Phase on Ischemia-Reperfusion Injury in a Rat Model of Non-Heart-Beating Donor Lung Transplantation.
      aug:
        au:
          Yan, Junwei
          Zhang, Bing
          Yu, Junmin
          Wang, Xuan
          Zhao, Han
          Li, Guangqi
          Yin, Yanwei
          Meng, Chao
        affil: Department of Vascular Surgery, The Affiliated Hospital of Qingdao University, Qingdao, China, 266000
      sug:
        subj:
          Ischemia Drug Therapy
          Reperfusion Injury Drug Therapy
          Hydrogen Sulfide Pharmacodynamics
          Lung Transplantation
          Transplant Donors
          Respiration, Artificial
          Animal Studies
          Rats
          Hydrogen Sulfide Administration and Dosage
          Time Factors
          Pulmonary Surfactants
          Bronchoalveolar Lavage
          Inflammation
          Lung Injury
          Comparative Studies
          Outcome Assessment
          Oxygenation Drug Effects
          Apoptosis Drug Effects
          Analysis of Variance
          Kruskal-Wallis Test
          Data Analysis Software
          Descriptive Statistics
          Funding Source
      ab: Donor lung ventilation and inflation during the warm ischemia could attenuate ischemia-reperfusion injury (IRI) after lung transplantation. Hydrogen sulfide (H2S), as a kind of protective gas, has demonstrated the antilung IRI effect. This study is aimed at observing the different methods of administering H2S in the setting of warm ischemia, ventilation, and inflation on the lung graft from a rat non-heart-beating donor. After 1 h of cardiac arrest, donor lungs in situ were inflated with 80 ppm H2S (FS group), ventilated with 80 ppm H2S (VS group), or deflated (control group) for 2 h. Then, the lung transplantation was performed after 3 h cold ischemia. The rats without ischemia and reperfusion were in the sham group. Pulmonary surfactant in the bronchoalveolar lavage fluid was measured in donor lung. The inflammatory response, cell apoptosis, and lung graft function were assessed at 3 h after reperfusion. The lung injury was exacerbated in the control group, which was attenuated significantly after the H2S treatment. Compared with the FS group, the pulmonary surfactant in the donor was deteriorated, the lung oxygenation function was decreased, and the inflammatory response and cell apoptosis were increased in the graft in the VS group (P < 0.05). In conclusion, H2S inflation during the warm ischemia phase improved the function of lung graft via regulating pulmonary surfactant stability and decreased the lung graft IRI via decreasing the inflammatory response and cell apoptosis.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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