Cytokine-Like Protein 1 (CYTL1) as a Key Target of M-Stage Immune Infiltration in Stomach Adenocarcinoma.

Background. Stomach adenocarcinoma (STAD) has an extremely high fatality rate worldwide, and survival after metastasis is extremely poor. Cytokine-like protein 1 (CYTL1) has prognostic significance in various tumors. We aimed to explore the impact and underlying molecular mechanisms of CYTL1 in STAD...

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Publicado en:BioMed Research International pp. 1 - 13
Autores principales: Zhou, Fang, Lin, QianXia, Zheng, ZiHan
Formato: research tables/charts Journal Article
Publicado: Wiley-Blackwell 2/13/2023
Acceso en línea:Ver este registro en EBSCOhost
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      dt: 2/13/2023
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1155/2023/2926218
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        atl: Cytokine-Like Protein 1 (CYTL1) as a Key Target of M-Stage Immune Infiltration in Stomach Adenocarcinoma.
      aug:
        au:
          Zhou, Fang
          Lin, QianXia
          Zheng, ZiHan
        affil: Vascular Breast Surgery, Jiangxi Provincial People's Hospital, Nanchang, Jiangxi 330006, China
      sug:
        subj:
          Stomach Neoplasms Immunology
          Adenocarcinoma Immunology
          Proteins Metabolism
          Bioinformatics
          Stomach Neoplasms Prognosis
          Adenocarcinoma Prognosis
          Tumor Markers, Biological Metabolism
          Molecular Biology
          Immunity
          Human
          Gene Expression
          Data Analysis Software
          Descriptive Statistics
          Cancer Patients
          Comparative Studies
          Kaplan-Meier Estimator
          Overall Survival
          Disease Attributes
          Univariate Statistics
          Multivariate Analysis
          Cox Proportional Hazards Model
          ROC Curve
          Predictive Value of Tests
          Signal Transduction
          Correlation Coefficient
          Algorithms
          Logistic Regression
          Odds Ratio
          Sex Factors
      ab: Background. Stomach adenocarcinoma (STAD) has an extremely high fatality rate worldwide, and survival after metastasis is extremely poor. Cytokine-like protein 1 (CYTL1) has prognostic significance in various tumors. We aimed to explore the impact and underlying molecular mechanisms of CYTL1 in STAD through bioinformatics analysis. Methods. We used R software to analyze CYTL1 expression in STAD samples (n = 375) and normal samples (n = 32) in The Cancer Genome Atlas database. Kaplan–Meier analysis was used to verify the relationship between CYTL1 expression and overall survival (OS) and disease-specific survival (DSS) based on the clinical characteristics and subgroups of patients with STAD. Furthermore, univariate and multivariate Cox regression analyses were used to verify the outcome variables of OS and DSS in patients with STAD. Receiver operating characteristic curves were used to test the predictive power of CYTL1. The biological functions and signaling pathways of CYTL1 were determined using gene set enrichment analysis (GSEA), and the immune infiltration patterns of CYTL1 and correlation of immune-related markers were analyzed using single-sample GSEA (ssGSEA) and an estimate algorithm. Results. In our research, low CYTL1 expression (tumor vs. normal) was noted in patients with STAD. High CYTL1 expression was detrimental to OS and DSS and had good diagnostic performance (AUC = 0.731). In the subtype analysis of STAD, T3 and T4 stages, N0 and N1 stages, M0 stage, gender (female), and age (≤65 years) showed different performances between OS and DSS. Univariate and multivariate Cox analyses identified CYTL1 as an independent factor, and logistic regression analysis indicated that CYTL1 was associated with M stage (OR = 3.406) and sex (OR = 1.535). GSEA of the differential genes of CYTL1 showed the possible involvement of immunity. ssGSEA and estimation algorithms were used to further evaluate whether immune cells were closely related to CYTL1 expression, and many markers of immune cells also had statistical significance with the expression of CYTL1. Conclusion. CYTL1 may, thus, act as an independent prognostic factor for STAD and regulate STAD progression by affecting the immune microenvironment.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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