Detection of Virulence Factors and Antibiotic Resistance among Klebsiella pneumoniae Isolates from Iran.
The current study assessed the detection of virulence genes and drug resistance among Klebsiella pneumoniae isolates from Iran. During 2018 to 2020, 52 K. pneumoniae isolates were obtained from patients at Iran hospitals. By disk diffusion method, the antimicrobial susceptibility of K. pneumoniae is...
| Publicado en: | BioMed Research International pp. 1 - 8 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
2/14/2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=161876491&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 161876491 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 23146133 FT2T jtl: BioMed Research International issn: 23146133 maglogo: N pubinfo: dt: 2/14/2023 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 161876491 161876491 161876491 10.1155/2023/3624497 161876491 ppf: 1 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Detection of Virulence Factors and Antibiotic Resistance among Klebsiella pneumoniae Isolates from Iran. aug: au: Davoudabadi, Sara Goudarzi, Mehdi Hashemi, Ali affil: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran sug: subj: Drug Resistance, Microbial Iran Virulence Klebsiella Infections Microbiology Human Iran Disease Susceptibility Polymerase Chain Reaction Descriptive Statistics Genes Funding Source ab: The current study assessed the detection of virulence genes and drug resistance among Klebsiella pneumoniae isolates from Iran. During 2018 to 2020, 52 K. pneumoniae isolates were obtained from patients at Iran hospitals. By disk diffusion method, the antimicrobial susceptibility of K. pneumoniae isolates was assessed, and ESBL-producing K. pneumoniae isolates were detected by CDDT method. PCR analysis was done to detect virulence genes (iucB, iutA, iroN, kfu, allS, fimH, ybtS, mrkD, and entB); ESBL-encoding genes (blaTEM, blaPER, blaCTX-M, blaVEB, and blaSHV); and class D (blaOXA-48), class B (blaVIM, blaNDM, and blaIMP), and class A (blaKPC and blaGES) carbapenemase genes. Among all isolates, 84.6%, 13.5%, and 1.9% isolates were multidrug-resistant (MDR), extensively drug-resistant (XDR), and pandrug-resistant (PDR), respectively. Also, 84.6% were ESBL-producing and 71.2%, 53.8%, 40.4%, and 9.6% of all isolates were carrying blaTEM, blaSHV, blaCTX-M, and blaOXA-48 genes, respectively. Six isolates (11.5%) were positive for blaNDM gene. In contrast, no isolates were positive for the presence of blaKPC, blaIMP, and blaVIM. Virulence factor genes including iucB, iutA, iroN, kfu, allS, fimH, ybtS, mrkD, and entB were carried by 24%, 46.2%, 25%, 11.5%, 17.3%, 86.5%, 75%, 88.5%, and 100% isolates, respectively. This study evaluated the distribution and prevalence of virulence factor genes among K. pneumoniae isolates. The treatment of these infections is challenging due to the existence of particular virulence factors and the rise of antibiotic resistance. Therefore, the current study accentuates the necessity of finding new and efficient solutions for stopping the increase of antibiotic resistance. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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