Gastroprotective action of the extract of Corydalis yanhusuo in Helicobacter pylori infection and its bioactive component, dehydrocorydaline.
Helicobacter pylori (H. pylori) infection is a frequent chronic infection. Persistent infection is the strongest risk factor for developing gastric complications leading to gastric cancer. The antibiotic resistance of current anti- H. pylori drugs lead to the search for novel candidates from medicin...
| Publicado en: | Journal of Ethnopharmacology Vol. 307 |
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| Autores principales: | , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
May2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=162108651&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 162108651 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: May2023 vid: 307 pid: 1004 pub: Elsevier B.V. artinfo: ui: 162108651 162108651 162108651 10.1016/j.jep.2023.116173 162108651 ppct: 1 formats: tig: atl: Gastroprotective action of the extract of Corydalis yanhusuo in Helicobacter pylori infection and its bioactive component, dehydrocorydaline. aug: au: Wu, Hao Sun, Qingyue Dong, Huirong Qiao, Jiasen lin, Ying Yu, Chen Li, Yanni affil: School of Pharmacy, Binzhou Medical University, Yantai, 264003, China sug: subj: Plant Extracts Pharmacodynamics Plants, Medicinal Helicobacter Infections Drug Therapy Plant Components Animal Studies Mice In Vitro Studies Antiinfective Agents In Vivo Studies Immunoglobulins Gastric Mucosa Drug Effects Phytochemicals Chromatography, High Pressure Liquid ab: Helicobacter pylori (H. pylori) infection is a frequent chronic infection. Persistent infection is the strongest risk factor for developing gastric complications leading to gastric cancer. The antibiotic resistance of current anti- H. pylori drugs lead to the search for novel candidates from medicinal plants. Traditionally, Corydalis yanhusuo (Y.H. Chou & Chun C.Hsu) W.T. Wang ex Z.Y. Su & C.Y. Wu (Papaveraceae) has been used for the treatment of digestive system diseases in China. So, it's essential to explore and confirm the anti- H. pylori activity of C. yanhusuo and characterize the pharmacologically active compounds. This study aims to evaluate the efficacy of C. yanhusuo as complementary or alternative modes of treatment against H. pylori -related diseases and ascertain the active substances of C. yanhusuo to develop non-toxic, natural, and inexpensive products. C. yanhusuo was subjected to solid-liquid extraction with water (WECY), ethanol EECY), and chloroform (CECY). The extracts were screened by agar diffusion assay, the minimum inhibitory concentrations (MIC), the minimum bactericidal (MBC) for their in vitro antimicrobial activity, and by Berthelot reaction for urease inhibition. To assess the in vivo action, H. pylori -induced C57BL/6 mice were used to detect RUT biopsy, perform visual and histopathological analyses and evaluate IgG expression. Furthermore, we compared the anti- H. pylori activities of major alkaloids in CECY to identify the bioactive constituents. Among the three C. yanhusuo extracts, CECY showed the maximum in vitro antibacterial activity. Administration of CECY significantly inhibited the survival of H. pylori colonized in the gastric mucosa and alleviated gastric damage along with a reduction in the expression levels of IgG in H. pylori -infected mice. Berberine and dehydrocorydaline exhibited obvious anti- H. pylori activity with MIC of 25 and 12.5 μg/mL, respectively. C. yanhusuo extracts showed anti- H. pylori activity in different degrees. Among them, CECY showed significant anti- H. pylori , gastroprotective and anti-inflammatory activities in vivo and in vitro. Dehydrocorydalmine, an active alkaloid compound isolated from C. yanhusuo , warranted further investigation for its potential anti- H. pylori activity. [Display omitted] • CECY was the most efficient against H. pylori of the three extracts in vitro. • The therapeutic efficacy of CECY against in vivo H. pylori infection was evaluated. • The phytochemical and HPLC of CECY analyses were performed. • Dehydrocorydaline isolated from CYCE can be highlighted. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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