Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach.

Common to numerous differentiation pathways in vertebrate organisms is the regulation of key genes through epigenetic mechanisms. Less well studied is to what extent cells of a given differentiation state, but examined at different points within the life history of an organism, are distinct at the l...

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Publicado en:Journals of Gerontology Series A: Biological Sciences & Medical Sciences Vol. 59; no. 12; pp. 1234 - 1244
Autores principales: Russanova, Valya R., Hirai, Tazuko H., Tchernov, Andrei V., Howard, Bruce H.
Formato: Journal Article
Publicado: Oxford University Press / USA Dec2004
Acceso en línea:Ver este registro en EBSCOhost
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      pub: Oxford University Press / USA
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        10.1093/gerona/59.12.1234
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        atl: Mapping development-related and age-related chromatin remodeling by a high throughput ChIP-HPLC approach.
      aug:
        au:
          Russanova, Valya R.
          Hirai, Tazuko H.
          Tchernov, Andrei V.
          Howard, Bruce H.
        affil: National Institute for Child Health and Human Development, National Institutes of Health, Bethesda, Maryland
      sug:
        subj:
          Aging
          Chromosome Mapping
          Proteins Metabolism
          Chromatography, High Pressure Liquid
          Metabolism
          Genome, Human
          Polymerase Chain Reaction
          Coping Health Inventory for Parents
      ab: Common to numerous differentiation pathways in vertebrate organisms is the regulation of key genes through epigenetic mechanisms. Less well studied is to what extent cells of a given differentiation state, but examined at different points within the life history of an organism, are distinct at the level of the epigenome. A few instances of such variation have been reported, and it would be of considerable value to have at hand a means to characterize additional examples more efficiently. We describe an integrated approach to this task, and further present evidence for regions of age-related histone H4 acetylation change extending over tens to hundreds of kilobases. Broad similarity between two distinct regions of such change suggests a previously unsuspected link between developmental programs and aging.
      pubtype: Academic Journal
      doctype: Journal Article
      ougenre: Article
    language: English
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