Dicer Suppresses Hepatocellular Carcinoma via Interleukin-8 Pathway.
Background: Elevated level of interleukin-8 (IL-8) promotes hepatocellular carcinoma (HCC) development and contributes to poor prognosis. Previously, we have proved that Dicer inhibits HCC progression. In this study, we evaluated the potential interaction between IL-8 and Dicer as well as their infl...
| Publicado en: | Clinical Medicine Insights: Oncology pp. 1 - 8 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Sage Publications Inc.
4/7/2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=162973470&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 162973470 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 11795549 B3KT jtl: Clinical Medicine Insights: Oncology issn: 11795549 maglogo: Y pubinfo: dt: 4/7/2023 pid: 344 pub: Sage Publications Inc. place: Thousand Oaks, California artinfo: ui: 162973470 162973470 162973470 10.1177/11795549231161212 162973470 ppf: 1 ppct: 7 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Dicer Suppresses Hepatocellular Carcinoma via Interleukin-8 Pathway. aug: au: Han, Xin Wu, Jianhua Sha, Ziyue Lai, Ruixue Shi, Jianfei Mi, Lili Yin, Fei Guo, Zhanjun affil: Department of Gastroenterology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, P.R. China sug: subj: Esterases Interleukins Blood Carcinoma, Hepatocellular Drug Therapy Drug Interactions Evaluation Carcinoma, Hepatocellular Prognosis Animal Studies Gene Expression Wound Healing Physiology Flow Cytometry Descriptive Statistics ab: Background: Elevated level of interleukin-8 (IL-8) promotes hepatocellular carcinoma (HCC) development and contributes to poor prognosis. Previously, we have proved that Dicer inhibits HCC progression. In this study, we evaluated the potential interaction between IL-8 and Dicer as well as their influence on HCC. Methods: Hepatocellular carcinoma cells of SMMC-7721 were divided into 2 groups for subsequent analysis: pCMV-Dicer group for Dicer-overexpressing lentivirus transfected cells (pCMV-Dicer cells) and pCMV-NC group for empty lentivirus transfected cells (pCMV-NC cells). Cell Counting kit-8 (CCK8), wound healing, and transwell were used to evaluate the inhibitory effect of Dicer overexpression on proliferation, migration, and invasion of HCC cells. The level of IL-8 was measured by flow cytometry bead-based immunoassays. Male nude BALB/c mice injected with pCMV-Dicer or pCMV-NC cell suspensions was used for transplant of HCC tumor. Results: We found that the secretion of IL-8 was reduced in the medium of pCMV-Dicer cells (P =.027). Recombinant human IL-8 (rhIL-8) reversed the inhibitory effect of Dicer on proliferation (P <.01), migration (P =.003), and invasion (P =.001), whereas IL-8 inhibitor of reparixin enhanced inhibitory effect of Dicer on proliferation (P <.05), migration (P =.008), and invasion (P =.000). Lenvatinib downregulated the IL-8 level of HCC cells (P =.000) as well as promote Dicer-induced inhibition for HCC cells referring to proliferation (P <.05), migration (P =.000), and invasion (P =.000). Animal experiments also demonstrated that Dicer cooperated with lenvatinib to inhibit the growth of HCC tumors (P <.05). Conclusions: Dicer cooperated with lenvatinib to inhibit HCC growth via downregulating IL-8, and Dicer displayed its potential capability to enhance the anti-tumor effect of lenvatinib. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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