Purification of Protein by HIC: Mechanistic Modeling for Improved Understanding and Process Optimization.
Hydrophobic interaction chromatography (HIC) is a separation technique widely used for the purification of therapeutic proteins. The main leverages identified to operate HIC processes are solution pH and ionic strength. In this article, the authors show that simplistic mathematical models derived fr...
| Publicado en: | BioPharm International Vol. 36; no. 4; pp. 22 - 30 |
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| Autores principales: | , , , , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
MJH Life Sciences
Apr2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=162985993&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 162985993 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1542166X 5NE jtl: BioPharm International issn: 1542166X maglogo: N pubinfo: dt: Apr2023 vid: 36 iid: 4 pid: 54670 pub: MJH Life Sciences place: Cranbury, New Jersey artinfo: ui: 162985993 162985993 162985993 162985993 ppf: 22 ppct: 8 formats: fmt: @attributes: type: P tig: atl: Purification of Protein by HIC: Mechanistic Modeling for Improved Understanding and Process Optimization. aug: au: NICOUD, LUCRÈCE LE GUENNEC, YOHANN NICOUD, EDOUARD CARDILLO, ANTONIO LIETTA, ELENA INNOCENTI, ELISA PIERI, ALESSANDRO affil: Head of product Ypso-Facto, France sug: subj: Proteins Metabolism Chromatography Methods Chemistry, Physical Adsorption Evaluation Models, Biological Human Experimental Studies Cell Culture Techniques Data Analysis, Statistical Simulations Osmolar Concentration ab: Hydrophobic interaction chromatography (HIC) is a separation technique widely used for the purification of therapeutic proteins. The main leverages identified to operate HIC processes are solution pH and ionic strength. In this article, the authors show that simplistic mathematical models derived from statistical analysis cannot describe the impact of pH and ionic strength observed in HIC processes. A new mechanistic model accounting for the protein charge in solution (varying with pH) and the solution non-ideality (activity coefficient varying with the ionic strength) is proposed here. This simple model is shown to accurately describe experimental data and paves the way for numerical optimization of HIC processes. pubtype: Trade Publication doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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