Transcriptomic profiling of sex-specific olfactory neurons reveals subset-specific receptor expression in Caenorhabditis elegans.
The nematode Caenorhabditis elegans utilizes chemosensation to navigate an ever-changing environment for its survival. A class of secreted small-molecule pheromones, termed ascarosides, play an important role in olfactory perception by affecting biological functions ranging from development to behav...
| Publicado en: | Genetics Vol. 223; no. 4; pp. 1 - 13 |
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| Autores principales: | , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
Apr2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=163027232&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 163027232 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00166731 GNT jtl: Genetics issn: 00166731 maglogo: N pubinfo: dt: Apr2023 vid: 223 iid: 4 pid: 622 pub: Oxford University Press / USA artinfo: ui: 163027232 163027232 163027232 10.1093/genetics/iyad026 163027232 ppf: 1 ppct: 12 formats: tig: atl: Transcriptomic profiling of sex-specific olfactory neurons reveals subset-specific receptor expression in Caenorhabditis elegans. aug: au: Reilly, Douglas K. Schwarz, Erich M. Muirhead, Caroline S. Robidoux, Annalise N. Narayan, Anusha Doma, Meenakshi K. Sternberg, Paul W. Srinivasan, Jagan affil: Department of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, MA 01605, USA. sug: subj: Gene Expression Profiling Sex Factors Neurons Physiology Gene Expression Chemoreceptor Cells Physiology Caenorhabditis Elegans Receptors, G-Protein-Coupled Metabolism Animal Studies Behavior, Animal CRISPR-Associated Proteins Metabolism Biological Factors Blood Smell Male Female Neurophysiology Funding Source Male Female ab: The nematode Caenorhabditis elegans utilizes chemosensation to navigate an ever-changing environment for its survival. A class of secreted small-molecule pheromones, termed ascarosides, play an important role in olfactory perception by affecting biological functions ranging from development to behavior. The ascaroside #8 (ascr#8) mediates sex-specific behaviors, driving avoidance in hermaphrodites and attraction in males. Males sense ascr#8 via the ciliated male-specific cephalic sensory (CEM) neurons, which exhibit radial symmetry along dorsal–ventral and left–right axes. Calcium imaging studies suggest a complex neural coding mechanism that translates stochastic physiological responses in these neurons to reliable behavioral outputs. To test the hypothesis that neurophysiological complexity arises from differential expression of genes, we performed cell-specific transcriptomic profiling; this revealed between 18 and 62 genes with at least twofold higher expression in a specific CEM neuron subtype vs both other CEM neurons and adult males. These included two G protein–coupled receptor (GPCR) genes, srw-97 and dmsr-12, that were specifically expressed in nonoverlapping subsets of CEM neurons and whose expression was confirmed by GFP reporter analysis. Single CRISPR-Cas9 knockouts of either srw-97 or dmsr12 resulted in partial defects, while a double knockout of both srw-97 and dmsr-12 completely abolished the attractive response to ascr#8. Together, our results suggest that the evolutionarily distinct GPCRs SRW-97 and DMSR-12 act nonredundantly in discrete olfactory neurons to facilitate male-specific sensation of ascr#8. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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