Within-Subject Variability and the Influence of Exercise Training History on the Resting Plasma Metabolome in Men.
This study investigated within-subject variability in the circulating metabolome under controlled conditions, and whether divergent exercise training backgrounds were associated with alterations in the circulating metabolome assessed in resting samples. Thirty-seven men comprising of endurance athle...
| Publicado en: | International Journal of Sport Nutrition & Exercise Metabolism Vol. 33; no. 3; pp. 141 - 151 |
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| Autores principales: | , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Human Kinetics Publishers, Inc.
5/1/2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=163255426&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 163255426 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 1526484X IJS jtl: International Journal of Sport Nutrition & Exercise Metabolism issn: 1526484X maglogo: N pubinfo: dt: 5/1/2023 vid: 33 iid: 3 pid: 553 pub: Human Kinetics Publishers, Inc. place: Champaign, Illinois artinfo: ui: 163255426 163255426 163255426 10.1123/ijsnem.2022-0177 163255426 ppf: 141 ppct: 10 formats: tig: atl: Within-Subject Variability and the Influence of Exercise Training History on the Resting Plasma Metabolome in Men. aug: au: Darragh, Ian A. J. O'Driscoll, Lorraine Egan, Brendan affil: School of Health and Human Performance, Dublin City University, Glasnevin, Ireland sug: subj: Exercise Relaxation Plasma Metabolites Physiology Exercise Physiology Human Funding Source Endurance Sports Athletes Food Intake Chromatography, Liquid Mass Spectrometry Intraclass Correlation Coefficient Factor Analysis Univariate Statistics Reliability Descriptive Statistics Comparative Studies Male Male ab: This study investigated within-subject variability in the circulating metabolome under controlled conditions, and whether divergent exercise training backgrounds were associated with alterations in the circulating metabolome assessed in resting samples. Thirty-seven men comprising of endurance athletes (END; body mass, 71.0 ± 6.8 kg; fat-free mass index, 16.9 ± 1.1 kg/m²), strength athletes (STR; 94.5 ± 8.8 kg; 23.0 ± 1.8 kg/m²), and recreationally active controls (CON; 77.6 ± 7.7 kg; 18.1 ± 1.0 kg/m²) provided blood samples after an overnight fast on two separate occasions controlled for time of day of sampling, recent dietary intake, time since last meal, and time since last exercise training session. A targeted profile of metabolites, performed using liquid chromatography and mass spectrometry on plasma samples, identified 166 individual metabolites and metabolite features, which were analyzed with intraclass correlation coefficients, a multilevel principal component analysis, and univariate t tests adjusted for multiple comparisons. The median intraclass correlation coefficient was .49, with 46 metabolites displaying good reliability and 31 metabolites displaying excellent reliability. No difference in the abundance of any individual metabolite was identified within groups when compared between visits, but a combined total of 44 metabolites were significantly different (false discovery rate <0.05) between groups (END vs. CON, 42 metabolites; STR vs. CON, 10 metabolites; and END vs. STR, five metabolites). Under similar measurement conditions, the reliability of resting plasma metabolite concentrations varies largely at the level of individual metabolites with ~48% of metabolites displaying good-to-excellent reliability. However, a history of exercise training was associated with alterations in the abundance of ~28% of metabolites in the targeted profile employed in this study. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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