In vivo transcriptome of Plasmodium falciparum reveals overexpression of transcripts that encode surface proteins.
Infections with the human parasite Plasmodium falciparum continue to present a great challenge to global health. Fundamental questions regarding the molecular basis of virulence and immune evasion in P. falciparum have been only partially answered. Because of the parasite's intracellular location an...
| Publicado en: | Journal of Infectious Diseases Vol. 191; no. 7; pp. 1196 - 1204 |
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| Autores principales: | , , , , , , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
Oxford University Press / USA
4/1/2005
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=16345817&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 16345817 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00221899 1YU jtl: Journal of Infectious Diseases issn: 00221899 maglogo: N pubinfo: dt: 4/1/2005 vid: 191 iid: 7 pid: 622 pub: Oxford University Press / USA artinfo: ui: 16345817 16345817 NLM15747257 16345817 10.1086/428289 NLM15747257 PMC2582152 16345817 ppf: 1196 ppct: 8 formats: tig: atl: In vivo transcriptome of Plasmodium falciparum reveals overexpression of transcripts that encode surface proteins. aug: au: Daily, Johanna P. Le Roch, Karine G. Sarr, Ousmane Ndiaye, Daouda Lukens, Amanda Yingyao Zhou Ndir, Omar Mboup, Soulyemane Sultan, Ali Winzeler, Elizabeth A. Wirth, Dyann F. Zhou, Yingyao affil: Department of Immunology and Infectious Disease, Harvard School of Public Health, Boston, Massachusetts sug: subj: Gene Expression Profiling Membrane Proteins Genes Protozoa Proteins Malaria RNA RNA Analysis Amino Acids Biological Phenomena Animals Oligonucleotide Array Sequence Analysis Child Adaptation, Physiological Adult Female Male Documentation Scales Funding Source Human Child: 6-12 years Adult: 19-44 years Female Male ab: Infections with the human parasite Plasmodium falciparum continue to present a great challenge to global health. Fundamental questions regarding the molecular basis of virulence and immune evasion in P. falciparum have been only partially answered. Because of the parasite's intracellular location and complex life cycle, standard genetic approaches to the study of the pathogenesis of malaria have been limited. The present study presents a novel approach to the identification of the biological processes involved in host-pathogen interactions, one that is based on the analysis of in vivo P. falciparum transcripts. We demonstrate that a sufficient quantity of P. falciparum RNA transcripts can be derived from a small blood sample from infected patients for whole-genome microarray analysis. Overall, excellent correlation was observed between the transcriptomes derived from in vivo samples and in vitro samples with ring-stage P. falciparum 3D7 reference strain. However, gene families that encode surface proteins are overexpressed in vivo. Moreover, this analysis has identified a new family of hypothetical genes that may encode surface variant antigens. Comparative studies of the transcriptomes derived from in vivo samples and in vitro 3D7 samples may identify important strategies used by the pathogen for survival in the human host and highlight, for vaccine development, new candidate antigens that were not previously identified through the use of in vitro cultures. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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