Co-infection of COVID-19 patients with atypical bacteria: A study based in Jordan.

Objective: The aim of this work was to know the prevalence of Chlamydophila pneumoniae and Mycoplasma pneumoniae in coronavirus disease 2019 (COVID-19) patients in Jordan. Also, to assess a TaqMan real-time polymerase chain reaction (PCR) assay in detecting these two bacteria. Methods: This is a ret...

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Published in:Pharmacy Practice (1886-3655) Vol. 21; no. 1; pp. 1 - 6
Main Authors: Alsayed, Ahmad R., Hasoun, Luai, Khader, Heba A., Abu-Samak, Mahmoud S., Al-Shdifat, Laith M. H., Al-shammari, Basheer, Al Maqbali, Mohammed
Format: research tables/charts Journal Article
Published: Centro de Investigaciones y Publicaciones Farmaceuticas S.L. Jan-Mar2023
Online Access:View this record in EBSCOhost
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        10.18549/PharmPract.2023.1.2753
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        atl: Co-infection of COVID-19 patients with atypical bacteria: A study based in Jordan.
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        au:
          Alsayed, Ahmad R.
          Hasoun, Luai
          Khader, Heba A.
          Abu-Samak, Mahmoud S.
          Al-Shdifat, Laith M. H.
          Al-shammari, Basheer
          Al Maqbali, Mohammed
        affil: Department of Clinical Pharmacy and Therapeutics, Faculty of Pharmacy, Applied Science Private University, Amman 11931-166, Jordan
      sug:
        subj:
          COVID-19
          Coinfection Epidemiology
          Chlamydophila Infections Epidemiology
          Pneumonia, Mycoplasma Epidemiology
          Polymerase Chain Reaction Methods
          Chlamydophila Infections Diagnosis
          Pneumonia, Mycoplasma Diagnosis
          Human
          Jordan
          Retrospective Design
          Nasopharynx Microbiology
          Chlamydophila Pneumoniae Analysis
          Mycoplasma Analysis
          Bacterial Proteins Analysis
          Correlation Coefficient
          Descriptive Statistics
          Prevalence
      ab: Objective: The aim of this work was to know the prevalence of Chlamydophila pneumoniae and Mycoplasma pneumoniae in coronavirus disease 2019 (COVID-19) patients in Jordan. Also, to assess a TaqMan real-time polymerase chain reaction (PCR) assay in detecting these two bacteria. Methods: This is a retrospective study performed over the last five months of the 2021. All nasopharyngeal specimens from COVID-19 patients were tested for C. pneumonia, and M. pneumoniae. The C. pneumoniae Pst-1 gene and M. pneumoniae P1 cytadhesin protein gene were the targets. Results: In this study, 14 out of 175 individuals with confirmed severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection (8.0%) were co-infected with C. pneumoniae or M. pneumoniae. Co-infection with SARS-CoV-2 and C. pneumoniae was reported in 5 (2.9%) patients, while 9 (5.1%) patients had M. pneumoniae and SARS-CoV-2 co-infection. The mean (± std) of the correlation coefficient of the calibration curve for real-time PCR analysis was -0.993 (± 0.001) for C. pneumoniae and -0.994 (± 0.003) for M. pneumoniae. The mean amplification efficiencies of C. pneumoniae and M. Pneumoniae were 187.62% and 136.86%, respectively. Conclusion: In this first study based in Jordan, patients infected with COVID-19 have a low rate of atypical bacterial co-infection. However, clinicians should suspect co-infections with both common and uncommon bacteria in COVID-19 patients. Large prospective investigations are needed to give additional insight on the true prevalence of these co-infections and their impact on the clinical course of COVID-19 patients.
      pubtype: Academic Journal
      doctype:
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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