Identification of the characteristics of infiltrating immune cells in pulpitis and its potential molecular regulation mechanism by bioinformatics method.
Objective: The inflammation of dental pulp will also trigger an immune response. The purpose of this study is to demonstrate the immune cell's function and explore their regulatory molecules and signal pathways in pulpitis. Method: The CIBERSORTx method was used to quantitatively analyze 22 types of...
| Publicado en: | BMC Oral Health Vol. 23; no. 1; pp. 1 - 16 |
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| Autores principales: | , , , , , , |
| Formato: | research tables/charts Journal Article |
| Publicado: |
BioMed Central
5/13/2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=163718125&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 163718125 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14726831 1CIC jtl: BMC Oral Health issn: 14726831 maglogo: N pubinfo: dt: 5/13/2023 vid: 23 iid: 1 pid: 24147 pub: BioMed Central artinfo: ui: 163718125 163718125 163718125 10.1186/s12903-023-03020-z 163718125 ppf: 1 ppct: 15 formats: tig: atl: Identification of the characteristics of infiltrating immune cells in pulpitis and its potential molecular regulation mechanism by bioinformatics method. aug: au: Wang, Jing Qiao, Junxia Ma, Lili Li, Xin Wei, Chengshi Tian, Xiufen Liu, Kun affil: Department of Stomatology, Liaocheng People's Hospital, 67 Dongchang West Road, 252000, Liaocheng, Shandong, P.R. China sug: subj: Dental Pulp Diseases Therapy Immune System Physiology Signal Transduction Bioinformatics Genes Human Quantitative Studies Blood Proteins Interleukins Descriptive Statistics Algorithms Tumor Necrosis Factor Neutrophils ab: Objective: The inflammation of dental pulp will also trigger an immune response. The purpose of this study is to demonstrate the immune cell's function and explore their regulatory molecules and signal pathways in pulpitis. Method: The CIBERSORTx method was used to quantitatively analyze 22 types of immune cells infiltrating in the GSE77459 dataset of dental pulp tissues. The immune-related differential genes (IR-DEGs) were further screened and enriched for the GO and KEGG pathways. Protein–protein interaction (PPI) networks were constructed and the hub IR-DEGs were screened. Finally, we constructed the regulatory network of hub genes. Results: The GSE77459 dataset screened 166 IR-DEGs and was enriched for three signal pathways involved in pulpitis development: chemokine signaling, TNF signaling, and NF-κB signaling. Significant differences in immune cell infiltration were observed between normal and inflamed dental pulp. The proportions of M0 macrophages, neutrophils, and follicular helper T cells were significantly higher than that of the normal dental pulp, while the proportions of resting mast cells, resting dendritic cells, CD8 T cells, and monocytes were significantly lower. The random forest algorithm concluded that M0 macrophages and neutrophils were the two most important immune cells. We identified five immune-related hub genes IL-6, TNF-α, IL-1β, CXCL8, and CCL2. In addition, IL-6, IL-1β, and CXCL8 are highly correlated with M0 macrophages and neutrophils, and the five hub genes have many shared regulatory molecules: four miRNAs and two lncRNAs, three transcription factors. Conclusion: Immune cell infiltration plays an important role in pulpitis among which M0 macrophages and neutrophils are the most significant immune cells. IL-6, TNF-α, IL-1, CXCL8, and CCL2 may be essential molecule of the immune response regulation network in pulpitis. This will help us understand the immune regulatory network in pulpitis. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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