Leukocyte deep learning classification assessment using Shapley additive explanations algorithm.

Introduction: A peripheral blood smear is a basic test for hematological disease diagnosis. This test is performed manually in many places worldwide, which requires both time and qualified staff. Large laboratories are equipped with digital morphology analyzers, some of which are based on deep learn...

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Publicado en:International Journal of Laboratory Hematology Vol. 45; no. 3; pp. 297 - 303
Autores principales: Michalski, Adrian, Duraj, Konrad, Kupcewicz, Bogumiła
Formato: pictorial research tables/charts Journal Article
Publicado: Wiley-Blackwell Jun2023
Acceso en línea:Ver este registro en EBSCOhost
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      jtl: International Journal of Laboratory Hematology
      issn: 17515521
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      dt: Jun2023
      vid: 45
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      pub: Wiley-Blackwell
      place: Malden, Massachusetts
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        10.1111/ijlh.14031
        163742613
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        atl: Leukocyte deep learning classification assessment using Shapley additive explanations algorithm.
      aug:
        au:
          Michalski, Adrian
          Duraj, Konrad
          Kupcewicz, Bogumiła
        affil: Department of Analytical Chemistry, Faculty of Pharmacy, University of Nicolaus Copernicus, Collegium Medicum, Bydgoszcz, Poland
      sug:
        subj:
          Leukocytes Classification
          Deep Learning Methods
          Algorithms
          Neural Networks (Computer) Methods
          Human
          Comparative Studies
          Cell Nucleus
          Laboratories
          Hematologic Tests
          Cytoplasm
      ab: Introduction: A peripheral blood smear is a basic test for hematological disease diagnosis. This test is performed manually in many places worldwide, which requires both time and qualified staff. Large laboratories are equipped with digital morphology analyzers, some of which are based on deep learning methods. However, it is difficult to explain to scientists how they work. In this paper, we proposed to add an explanatory factor to enhance the interpretability of deep learning models in leukocyte classification. Methods: 10 297 single images of leukocytes obtained from peripheral blood smears were included in this study. Pre‐trained and fully trained VGG16 and VGG19 models were used to classify the leukocytes, and Shapley Additive Explanations (SHAP) DeepExplainer was applied to visualize the area of cells that were significant for classification. The output images from the DeepExplainer were compared with cellular elements that are essential to laboratory practice. Results: The accuracy of our fully trained models was 99.81% for VGG16 and 99.79% for VGG19. It achieved slightly better results than the partially trained model, which scored 98.67% for VGG16 and 98.33% for VGG19. Their SHAP explanations indicated the significance of cellular structures in microscopic examination. Explanations in the pre‐trained models have proved the cell and nucleus contours to be relevant to classification, while explanations in the fully trained models pointed to the cytoplasm area. Conclusion: Despite different SHAP DeepExplainer explanations for fully and partially trained models, this method appears to be helpful for the verification of leukocyte classification in automated peripheral blood smear examination.
      pubtype: Academic Journal
      doctype:
        pictorial
        research
        tables/charts
        Journal Article
      ougenre: Article
    language: English
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