The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression.
Antisense transcript of the B-cell translocation gene 3 (ASBEL) is a highly conserved antisense non-coding RNA (ncRNA) and participates in a variety of biological processes. However, the ASBEL expression status in pancreatic ductal adenocarcinoma (PDAC) and its correlation with BTG3 expression and t...
| Publicado en: | Current Therapeutic Research Vol. 98 |
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| Autores principales: | , , , , , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=164402091&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 164402091 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0011393X KIH jtl: Current Therapeutic Research issn: 0011393X maglogo: N pubinfo: dt: 2023 vid: 98 pid: 467 pub: Elsevier B.V. place: New York, New York artinfo: ui: 164402091 164402091 164402091 10.1016/j.curtheres.2023.100700 164402091 ppct: 1 formats: tig: atl: The Role of Noncoding RNA Antisense Transcript of the B-Cell Translocation Gene 3 Regulation of BTG3 in Pancreatic Ductal Adenocarcinoma Tumor Progression. aug: au: Chen, Jing Zhu, Ming-Yuan Huang, Yan-Hua Ling, Yi-Ting Gu, Tian-Yuan Zhou, Quan Fei, Ming-Jian Zhou, Zhong-Cheng affil: Department of General Surgery, The Second Affiliated Hospital of Jiaxing University, Zhejiang Province, China sug: subj: Pancreatic Neoplasms Familial and Genetic Carcinoma, Ductal Familial and Genetic Pancreatic Neoplasms Physiopathology Carcinoma, Ductal Physiopathology Disease Progression Physiopathology RNA Physiology B Lymphocytes Gene Expression Profiling Human Animal Studies In Vitro Studies In Vivo Studies Blotting, Western Polymerase Chain Reaction Cell Count Flow Cytometry Cell Proliferation Apoptosis Neoplasm Invasiveness Cell Movement Xenografts Mice Models, Biological Tumor Markers, Biological ab: Antisense transcript of the B-cell translocation gene 3 (ASBEL) is a highly conserved antisense non-coding RNA (ncRNA) and participates in a variety of biological processes. However, the ASBEL expression status in pancreatic ductal adenocarcinoma (PDAC) and its correlation with BTG3 expression and tumor cell progression were not completely clear. We conducted cell experiments and animal experiments to confirm that ASBEL plays a crucial role in the tumorigenesis of PDAC by targeting BTG3. ASBEL regulation in PDAC tumorigenesis was evaluated using Western blotting, quantitative polymerase chain reaction, Cell Counting Kit-8 assay, flow cytometry, and cell transfection. We also evaluated the expression of ASBEL and BTG3 in tumor tissues and cells using Western blotting and quantitative real-time polymerase chain reaction. Finally, we explored the role of ASBEL in tumor development by silencing or overexpressing ASBEL gene in AsPC-1 or CFPAC-1 cells, respectively, and evaluated the antitumor activity in vivo using an ASBEL antagonist. Our study revealed the expression of ASBEL in all pancreatic cell lines. The expression level of ASBEL in tumor tissues was found to be higher than that of paracarcinomatous tissues. ASBEL suppresses expression of BTG3 , enhances proliferation and suppresses apoptosis, and promotes migration and invasion in pancreatic cancer cell. Antagonist regulates the expression of ASBEL in AsPC-1, and suppresses tumor growth in xenograft mouse model. Our results indicate that ASBEL may play a tumor-promoting factor in PDAC by targeting BTG3 and could be as an important biomarker for PDAC treatment. (Curr Ther Res Clin Exp. 2023; 84:XXX–XXX). pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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