Comprehensive transcript-level analysis reveals transcriptional reprogramming during the progression of Alzheimer's disease.
Background: Alzheimer's disease (AD) is a common neurodegenerative disorder that has a multi-step disease progression. Differences between moderate and advanced stages of AD have not yet been fully characterized. Materials and methods: Herein, we performed a transcript-resolution analysis in 454 AD-...
| Publicado en: | Frontiers in Aging Neuroscience pp. 1 - 12 |
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| Autores principales: | , , , , , , , |
| Formato: | equations & formulas research tables/charts Journal Article |
| Publicado: |
Frontiers Media S.A.
2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=164605173&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 164605173 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 16634365 BG2U jtl: Frontiers in Aging Neuroscience issn: 16634365 maglogo: N pubinfo: dt: 2023 pid: 40038 pub: Frontiers Media S.A. artinfo: ui: 164605173 164605173 164605173 10.3389/fnagi.2023.1191680 164605173 ppf: 1 ppct: 11 formats: tig: atl: Comprehensive transcript-level analysis reveals transcriptional reprogramming during the progression of Alzheimer's disease. aug: au: Hao Wu Jiao Wang Xiaoyuan Hu Cheng Zhuang Jianxin Zhou Peiru Wu Shengli Li Chunhua Zhao, Robert affil: Laboratory of Molecular Neural Biology, School of Life Sciences, Shanghai University, Shanghai, China sug: subj: Alzheimer's Disease Disease Progression Gene Expression Profiling Human Descriptive Statistics Data Analysis Software Spearman's Rank Correlation Coefficient Funding Source Comparative Studies RNA-Binding Proteins Metabolism Biological Markers Pearson's Correlation Coefficient Early Diagnosis ab: Background: Alzheimer's disease (AD) is a common neurodegenerative disorder that has a multi-step disease progression. Differences between moderate and advanced stages of AD have not yet been fully characterized. Materials and methods: Herein, we performed a transcript-resolution analysis in 454 AD-related samples, including 145 non-demented control, 140 asymptomatic AD (AsymAD), and 169 AD samples. We comparatively characterized the transcriptome dysregulation in AsymAD and AD samples at transcript level. Results: We identified 4,056 and 1,200 differentially spliced alternative splicing events (ASEs) that might play roles in the disease progression of AsymAD and AD, respectively. Our further analysis revealed 287 and 222 isoform switching events in AsymAD and AD, respectively. In particular, a total of 163 and 119 transcripts showed increased usage, while 124 and 103 transcripts exhibited decreased usage in AsymAD and AD, respectively. For example, gene APOA2 showed no expression changes between AD and non-demented control samples, but expressed higher proportion of transcript ENST00000367990.3 and lower proportion of transcript ENST00000463812.1 in AD compared to non-demented control samples. Furthermore, we constructed RNA binding protein (RBP)-ASE regulatory networks to reveal potential RBP-mediated isoform switch in AsymAD and AD. Conclusion: In summary, our study provided transcript-resolution insights into the transcriptome disturbance of AsymAD and AD, which will promote the discovery of early diagnosis biomarkers and the development of new therapeutic strategies for patients with AD. pubtype: Academic Journal doctype: equations & formulas research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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