Type I interferon and neutrophil transcripts in lupus nephritis renal biopsies: clinical and histopathological associations.
Objectives To investigate the expression of type I IFN (IFN-I) and neutrophil transcripts in kidney tissue from patients with different classes of LN and their association with distinct clinical and histopathological features. Methods Quantitation of IFN-I, defensin-α3 and formyl peptide receptor-li...
| Published in: | Rheumatology Vol. 62; no. 7; pp. 2534 - 2539 |
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| Main Authors: | , , , |
| Format: | research tables/charts Journal Article |
| Published: |
Oxford University Press / USA
Jul2023
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=164776469&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 164776469 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14620324 DN9 jtl: Rheumatology issn: 14620324 maglogo: N pubinfo: dt: Jul2023 vid: 62 iid: 7 pid: 622 pub: Oxford University Press / USA artinfo: ui: 164776469 164776469 164776469 10.1093/rheumatology/keac641 164776469 ppf: 2534 ppct: 5 formats: tig: atl: Type I interferon and neutrophil transcripts in lupus nephritis renal biopsies: clinical and histopathological associations. aug: au: Mavragani, Clio P Kirou, Kyriakos A Seshan, Surya V Crow, Mary K affil: Department of Medicine, Mary Kirkland Center for Lupus Research, Hospital for Special Surgery and Weill Cornell Medicine , New York, NY, USA sug: subj: Interferons Neutrophils Gene Expression Profiling Biopsy Lupus Nephritis Symptoms Disease Attributes Lupus Nephritis Pathology Human Funding Source Alarmins Receptors, Immunologic Glomerular Filtration Rate ab: Objectives To investigate the expression of type I IFN (IFN-I) and neutrophil transcripts in kidney tissue from patients with different classes of LN and their association with distinct clinical and histopathological features. Methods Quantitation of IFN-I, defensin-α3 and formyl peptide receptor-like 1 (FPRL-1) transcripts was performed in kidney biopsy tissue from 24 patients with various classes of LN (6 class III, 14 class IV, 4 class V) and 3 control samples. Patient demographics, glomerular filtration rate (eGFR) and histopathological characteristics, including activity and chronicity indices, were analysed. Results IFNα2 and IFNβ transcripts were overexpressed in renal tissues from patients with proliferative forms of LN (III/IV) compared with patients with membranous nephritis and control kidneys. Patients with LN and impaired renal function, attested by eGFR, displayed higher relative expression of IFNα2 transcripts in renal tissues compared with those with normal renal function (23.0 ± 16.2 vs 12.0 ± 14.8, P = 0.04). Defensin-α3, but not FPRL-1 , transcripts were overexpressed in LN tissues, particularly those with segmental necrotizing lesions, and were correlated with higher renal pathological activity indices (r = 0.61, P = 0.02), urinary protein levels (r = 0.44, P = 0.048) and IFNα2 expression (r = 0.50, P = 0.01). Conclusion IFN-I transcripts are expressed locally in kidneys from patients with proliferative LN and are associated with impaired renal function. Elevated defensin-α3 transcripts, a neutrophil product associated with neutrophil extracellular traps, may identify a driver of local IFN-I expression. These findings provide insights into the mechanisms of proliferative LN and may inform therapeutic decisions regarding selection of IFN-I pathway inhibitors. pubtype: Academic Journal doctype: research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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