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Investigation of D-Dimer stability with INNOVANCE D-Dimer assay.

Objectives: The present study aimed to examine whether the D-dimer stability time can be extended from 4 hours to 6 or 8 hours at room temperature to account for late arrival samples and add-on test requests received after 4 hours. Methods: Three citrate blood tubes were collected from 25 patients....

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Published in:New Zealand Journal of Medical Laboratory Science Vol. 77; no. 2; p. 95
Main Authors: Yan Fu, Yii Sen Wee
Format: abstract research Journal Article
Published: New Zealand Institute of Medical Laboratory Science Jul2023
Online Access:View this record in EBSCOhost
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      dt: Jul2023
      vid: 77
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      pub: New Zealand Institute of Medical Laboratory Science
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        atl: Investigation of D-Dimer stability with INNOVANCE D-Dimer assay.
      aug:
        au:
          Yan Fu
          Yii Sen Wee
        affil: University of Otago, Dunedin
      sug:
        subj:
          Fibrin Fibrinogen Degradation Products Analysis
          Time Factors
          Blood Preservation Methods
          Biological Assay
          Antifibrinolytic Agents
          Cryopreservation
          Centrifugation Methods
          Temperature
          Regression
      ab: Objectives: The present study aimed to examine whether the D-dimer stability time can be extended from 4 hours to 6 or 8 hours at room temperature to account for late arrival samples and add-on test requests received after 4 hours. Methods: Three citrate blood tubes were collected from 25 patients. For each patient, a baseline tube was centrifuged and tested for D-dimer upon arrival (within 4 hours). This baseline tube was then repeatedly tested at 6 hours and 8 hours post-sample collection. The other two tubes were stored horizontally at room temperature until 6 hours and 8 hours post-sample collection, at which they were centrifuged and tested, respectively. All tests were analysed with the INNOVANCE D-Dimer assay. Results: The D-dimer levels at baseline ranged from less than 190 μg/L FEU to 10512 μg/L FEU. The mean percentage changes at 6 hours and 8 hours were all below the clinically acceptable cut-off of 10% compared with the baseline. Specifically, for centrifuged samples, a decrease of 2.39% and 2.23% were found after 6 and 8 hours, respectively. For uncentrifuged samples, a decrease of 2.83% and 1.93% were observed at 6 and 8 hours, respectively. Results from each tested condition all met the allowed limit of performance in EFLM (European Federation of Clinical Chemistry and Laboratory Medicine). Further analysis using Passing-Bablock regression showed excellent agreement between D-dimer levels at baseline and D-dimer levels after 6-and 8-hours storage. Conclusion: Our study demonstrated that the stability of D-dimer at room temperature can be reliably extended to 8 hours for both centrifuged and uncentrifuged whole blood samples.
      pubtype: Academic Journal
      doctype:
        abstract
        research
        Journal Article
      ougenre: Article
    language: English
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