Autologous i‐PRF promotes healing of radiation‐induced skin injury.
Skin, as an exposed tissue, often suffers damage after exposure to radiotherapy and accidental events, which may lead to the formation of chronic refractory wounds. However, effective treatment options are usually limited for severe radiation‐induced skin injury (RSI). Platelet‐rich plasma (PRP) has...
| Publicado en: | Wound Repair & Regeneration Vol. 31; no. 4; pp. 454 - 464 |
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| Autores principales: | , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Wiley-Blackwell
Jul/Aug2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=165469988&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 165469988 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 10671927 DPV jtl: Wound Repair & Regeneration issn: 10671927 maglogo: Y pubinfo: dt: Jul/Aug2023 vid: 31 iid: 4 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 165469988 163880100 165469988 165469988 10.1111/wrr.13083 165469988 ppf: 454 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: C – @attributes: type: P tig: atl: Autologous i‐PRF promotes healing of radiation‐induced skin injury. aug: au: Tian, Kai Ye, Jingcheng Zhong, Yuanyuan Jia, Zou Xu, Wushuang Gao, Suyue Cao, Shikun Li, Ke Wu, Lijun affil: Department of Plastic and Aesthetic Surgery, The Second Affiliated Hospital of Soochow University, Suzhou, China sug: subj: Skin Injuries Radiation Injuries Therapy Platelet-Rich Fibrin Transplantation Platelet-Rich Plasma Transplantation Blood Transfusion, Autologous Wound Healing Evaluation Treatment Outcomes Human Animal Studies Rats Funding Source In Vitro Studies In Vivo Studies Endothelial Cells Cell Migration Assays Apoptosis Radiation Effects Cell Viability Radiation Effects Neovascularization, Physiologic Reactive Oxygen Species Radiation Effects Staining and Labeling Inflammation Prevention and Control ab: Skin, as an exposed tissue, often suffers damage after exposure to radiotherapy and accidental events, which may lead to the formation of chronic refractory wounds. However, effective treatment options are usually limited for severe radiation‐induced skin injury (RSI). Platelet‐rich plasma (PRP) has been identified to promote wound healing, but whether a new generation of blood‐derived biomaterial, injectable platelet‐rich fibrin (i‐PRF), is effective in repairing RSI remains unclear. In this study, blood was drawn from humans and Sprague–Dawley rats to prepare PRP and i‐PRF, and the regenerative functions of PRP and i‐PRF were investigated by exposing the dorsal skin of SD rats to local radiation (45 Gy) and exposing HDF‐α cells and human umbilical vein endothelial cells (HUVECs) cells to X‐rays (10 Gy). The healing effect of i‐PRF on RSI was analysed by tube formation assay, cell migration and apoptosis assays, ROS assay, wound healing assay, histological characterisation and immunostaining. The results showed that exposure to high doses of radiation reduced cell viability, increased ROS levels and induced cell apoptosis, thereby causing dorsal trauma of rats. However, both PRP and i‐PRF could resisted RSI, and they were capable of reducing inflammation and promoting angiogenesis and vascular regeneration. i‐PRF has a higher concentration of platelets and platelet‐derived growth factors, which has a more convenient preparation method and better repair effect and possesses a good application prospect for the repair of RSI. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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