Synchrotron micro-computed tomography unveils the three-dimensional structure and origin of staminodes in the Plains Prickly Pear Cactus Opuntia polyacantha Haw. (Cactaceae).
Floral appendages display an array of shapes and sizes. Among these organs, staminodes are morphologically diverse structures that have lost the ability to produce pollen, but in some instances, they produce fertile pollen grains. In the family Cactaceae staminodes are uncommon and range from simple...
| Publicado en: | Protoplasma Vol. 260; no. 5; pp. 1303 - 1313 |
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| Autores principales: | , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Springer Nature
Sep2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=169781316&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 169781316 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 0033183X 2CF jtl: Protoplasma issn: 0033183X maglogo: N pubinfo: dt: Sep2023 vid: 260 iid: 5 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 169781316 162289694 10.1007/s00709-023-01846-6 169781316 ppf: 1303 ppct: 10 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Synchrotron micro-computed tomography unveils the three-dimensional structure and origin of staminodes in the Plains Prickly Pear Cactus Opuntia polyacantha Haw. (Cactaceae). aug: au: Cota-Sánchez, J. Hugo Falconer, Denver J. de Almeida, Odair J. G. Stobbs, Jarvis A. Vera-Vélez, Roy Rice, Ryan S. Belliveau, Nicholas A. affil: Department of Biology, University of Saskatchewan, 112 Science Place, S7N 5E2, Saskatoon, SK, Canada sug: ab: Floral appendages display an array of shapes and sizes. Among these organs, staminodes are morphologically diverse structures that have lost the ability to produce pollen, but in some instances, they produce fertile pollen grains. In the family Cactaceae staminodes are uncommon and range from simple linear to flat to spatulate structures, but studies describing their structural attributes are scanty. This study highlights the advantages of synchrotron radiation for sample preparation and as a research tool for plant biology. It describes the internal morphology of floral parts, particularly stamen, tepal, and staminode in the Plains Prickly Pear Cactus, Opuntia polyacantha, using synchrotron radiation micro-computed tomography (SR-μCT). It also shows the different anatomical features in reconstructed three-dimensional imaging of reproductive parts and discuss the advantages of the segmentation method to detect and characterize the configuration and intricate patterns of vascular networks and associated structures of tepal and androecial parts applying SR-μCT. This powerful technology led to substantial improvements in terms of resolution allowing a more comprehensive understanding of the anatomical organization underlying the vasculature of floral parts and inception of staminodes in O. polyacantha. Tepal and androecial parts have uniseriate epidermis enclosing loose mesophyll with mucilage secretory ducts, lumen, and scattered vascular bundles. Cryptic underlying structural attributes provide evidence of a vascularized pseudo-anther conjoint with tepals. The undefined contours of staminodial appendages (pseudo-anther) amalgamated to the tepals' blurred boundaries suggest that staminodes originate from tepals, a developmental pattern supporting the fading border model of floral organ identity for angiosperms. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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