Multispectral Imaging Method for Rapid Identification and Analysis of Paraffin-Embedded Pathological Tissues.
The study of the interaction between light and biological tissue is of great help in the identification of diseases as well as structural alterations in tissues. In the present study, we have developed a tissue diagnostic technique by using multispectral imaging in the visible spectrum combined with...
| Publicado en: | Journal of Digital Imaging Vol. 36; no. 4; pp. 1663 - 1675 |
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| Autores principales: | , , , |
| Formato: | equations & formulas pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Aug2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=169808820&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 169808820 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 08971889 DOQ jtl: Journal of Digital Imaging issn: 08971889 maglogo: N pubinfo: dt: Aug2023 vid: 36 iid: 4 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 169808820 163162723 169808820 169808820 10.1007/s10278-023-00826-9 169808820 ppf: 1663 ppct: 12 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Multispectral Imaging Method for Rapid Identification and Analysis of Paraffin-Embedded Pathological Tissues. aug: au: Sijilmassi, Ouafa López Alonso, José-Manuel Del Río Sevilla, Aurora Barrio Asensio, María del Carmen affil: Faculty of Optics and Optometry, Anatomy and Embryology Department, Universidad Complutense de Madrid, Madrid, Spain sug: subj: Spectroscopy, Near-Infrared Methods Tissue Radiography Paraffin Embedding Tissue Pathology Animal Studies Mice Spain Embryo Eye Hemorrhage Image Processing, Computer Assisted Light Utilization Funding Source ab: The study of the interaction between light and biological tissue is of great help in the identification of diseases as well as structural alterations in tissues. In the present study, we have developed a tissue diagnostic technique by using multispectral imaging in the visible spectrum combined with principal component analysis (PCA). We used information from the propagation of light through paraffin-embedded tissues to assess differences in the eye tissues of control mouse embryos compared to mouse embryos whose mothers were deprived of folic acid (FA), a crucial vitamin necessary for the growth and development of the fetus. After acquiring the endmembers from the multispectral images, spectral unmixing was used to identify the abundances of those endmembers in each pixel. For each acquired image, the final analysis was performed by performing a pixel-by-pixel and wavelength-by-wavelength absorbance calculation. Non-negative least squares (NNLS) were used in this research. The abundance maps obtained for the first endmember revealed vascular alterations (vitreous and choroid) in the embryos with maternal FA deficiency. However, the abundance maps obtained for the third endmember showed alterations in the texture of some tissues such as the lens and retina. Results indicated that multispectral imaging applied to paraffin-embedded tissues enhanced tissue visualization. Using this method, first, it can be seen tissue damage location and then decide what kind of biological techniques to apply. pubtype: Academic Journal doctype: equations & formulas pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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