Salidroside suppresses the multiple oncogenic activates and immune escape of lung adenocarcinoma through the circ_0009624‐mediated PD‐L1 pathway.
Background: Lung adenocarcinoma (LUAD) is a fatal malignancy all over the world. Salidroside (SAL) is an active component extracted from Rhodiola rosea that has been reported to exert antitumor activity against several human cancers, containing lung adenocarcinoma (LUAD). The purpose of this study w...
| Published in: | Thoracic Cancer Vol. 14; no. 24; pp. 2493 - 2504 |
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| Main Authors: | , , , , |
| Format: | pictorial research tables/charts Journal Article |
| Published: |
Wiley-Blackwell
Aug2023
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| Online Access: | View this record in EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=170079295&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 170079295 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 17597706 BDCY jtl: Thoracic Cancer issn: 17597706 maglogo: Y pubinfo: dt: Aug2023 vid: 14 iid: 24 pid: 480 pub: Wiley-Blackwell place: Malden, Massachusetts artinfo: ui: 170079295 170079295 170079295 10.1111/1759-7714.15034 170079295 ppf: 2493 ppct: 11 formats: tig: atl: Salidroside suppresses the multiple oncogenic activates and immune escape of lung adenocarcinoma through the circ_0009624‐mediated PD‐L1 pathway. aug: au: Jin, Guilin Ma, Mi Yang, Chunyan Zhen, Luo Feng, Mingke affil: University of Tibetan Medicine, Lhasa, China sug: subj: Adenocarcinoma of Lung Drug Therapy Rhodiola Plant Extracts Pharmacodynamics Antineoplastic Agents Pharmacodynamics Cell Line, Tumor Drug Effects Human Cell Viability Cell Proliferation Neoplasm Invasiveness Lactate Dehydrogenase Flow Cytometry Blotting, Western Polymerase Chain Reaction Xenografts Phenotype Funding Source Programmed Cell Death Ligand 1 Signal Transduction Cell Movement ab: Background: Lung adenocarcinoma (LUAD) is a fatal malignancy all over the world. Salidroside (SAL) is an active component extracted from Rhodiola rosea that has been reported to exert antitumor activity against several human cancers, containing lung adenocarcinoma (LUAD). The purpose of this study was to explore the effect and underlying mechanism of SAL in LUAD. Methods: Cell viability, proliferation, migration, and invasion were measured using cell counting kit‐8 (CCK‐8), 5‐ethynyl‐2′‐deoxyuridine (EdU), and transwell assays. Effects of LUAD cells on the cytotoxicity, percentage, and death of CD8+ cells were detected using lactate dehydrogenase (LDH) and flow cytometry assays. Programmed cell death ligand 1 (PD‐L1) protein level was examined using western blot. Circ_0009624, enolase 1 (ENO1), and PD‐L1 levels were determined using real‐time quantitative polymerase chain reaction (RT‐qPCR). The biological role of SAL on LUAD tumor growth was assessed using the xenograft tumor model in vivo. Results: SAL restrained LUAD cell proliferation, migration, invasion, and immune escape in vitro via modulating PD‐L1. Circ_0009624 expression was increased in LUAD. Applying SAL repressed circ_0009624 and PD‐L1 expression in LUAD cells. SAL treatment hindered suppressed various oncogenic activates and immune escape of LUAD cells by regulating the circ_0009624/PD‐L1 pathway. SAL blocked LUAD xenograft growth in vivo. Conclusion: Applying SAL might constrain malignant phenotypes and immune escape of LUAD cells partially through the circ_0009624‐mediated PD‐L1 pathway, providing a novel insight for LUAD treatment. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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