Prebiotics modulate the microbiota–gut–brain axis and ameliorate cognitive impairment in APP/PS1 mice.
Purpose: Prebiotics, including fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS), stimulate beneficial gut bacteria and may be helpful for patients with Alzheimer's disease (AD). This study aimed to compare the effects of FOS and GOS, alone or in combination, on AD mice and to identif...
| Publicado en: | European Journal of Nutrition Vol. 62; no. 7; pp. 2991 - 3008 |
|---|---|
| Autores principales: | , , , , , , , , , |
| Formato: | pictorial research tables/charts Journal Article |
| Publicado: |
Springer Nature
Oct2023
|
| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=171101895&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 171101895 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 14366207 CR0 jtl: European Journal of Nutrition issn: 14366207 maglogo: N pubinfo: dt: Oct2023 vid: 62 iid: 7 pid: 237 pub: Springer Nature place: New York, New York artinfo: ui: 171101895 164956671 171101895 171101895 10.1007/s00394-023-03208-7 171101895 ppf: 2991 ppct: 17 formats: fmt: – @attributes: type: T – @attributes: type: P tig: atl: Prebiotics modulate the microbiota–gut–brain axis and ameliorate cognitive impairment in APP/PS1 mice. aug: au: Zhang, Shujuan Lv, Shuang Li, Yiming Wei, Dongfeng Zhou, Xiao Niu, Xiaoqian Yang, Ziyuan Song, Weiqun Zhang, Zhanjun Peng, Dantao affil: Department of Rehabilitation Medicine, Xuan Wu Hospital, Capital Medical University, 45 Chang Chun Street, 100053, Beijing, China sug: subj: Prebiotics Therapeutic Use Oligosaccharides Pharmacodynamics Gut Microbiota Metabolism Brain-Gut Axis Cognition Disorders Prevention and Control Models, Biological Animal Studies Rats Funding Source Neuropsychological Tests RNA Analysis Sequence Analysis Interleukins Drug Effects Lactobacillus Drug Effects Signal Transduction ab: Purpose: Prebiotics, including fructo-oligosaccharides (FOS) and galacto-oligosaccharides (GOS), stimulate beneficial gut bacteria and may be helpful for patients with Alzheimer's disease (AD). This study aimed to compare the effects of FOS and GOS, alone or in combination, on AD mice and to identify their underlying mechanisms. Methods: Six-month-old APP/PS1 mice and wild-type mice were orally administered FOS, GOS, FOS + GOS or water by gavage for 6 weeks and then subjected to relative assays, including behavioral tests, biochemical assays and 16S rRNA sequencing. Results: Through behavioral tests, we found that GOS had the best effect on reversing cognitive impairment in APP/PS1 mice, followed by FOS + GOS, while FOS had no effect. Through biochemical techniques, we found that GOS and FOS + GOS had effects on multiple targets, including diminishing Aβ burden and proinflammatory IL-1β and IL-6 levels, and changing the concentrations of neurotransmitters GABA and 5-HT in the brain. In contrast, FOS had only a slight anti-inflammatory effect. Moreover, through 16S rRNA sequencing, we found that prebiotics changed composition of gut microbiota. Notably, GOS increased relative abundance of Lactobacillus, FOS increased that of Bifidobacterium, and FOS + GOS increased that of both. Furthermore, prebiotics downregulated the expression levels of proteins of the TLR4-Myd88-NF-κB pathway in the colons and cortexes, suggesting the involvement of gut–brain mechanism in alleviating neuroinflammation. Conclusion: Among the three prebiotics, GOS was the optimal one to alleviate cognitive impairment in APP/PS1 mice and the mechanism was attributed to its multi-target role in alleviating Aβ pathology and neuroinflammation, changing neurotransmitter concentrations, and modulating gut microbiota. pubtype: Academic Journal doctype: pictorial research tables/charts Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
|---|