Digital PCR Partition Classification.
BACKGROUND: Partition classification is a critical step in the digital PCR data analysis pipeline. A range of partition classification methods have been developed, many motivated by specific experimental setups. An overview of these partition classification methods is lacking and their comparative p...
| Publicado en: | Clinical Chemistry Vol. 69; no. 9; pp. 976 - 991 |
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| Autores principales: | , , , , , , , |
| Formato: | Journal Article |
| Publicado: |
Oxford University Press / USA
Sep2023
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=171889248&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 171889248 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 00099147 10CS jtl: Clinical Chemistry issn: 00099147 maglogo: N pubinfo: dt: Sep2023 vid: 69 iid: 9 pid: 622 pub: Oxford University Press / USA artinfo: ui: 171889248 10.1093/clinchem/hvad063 171889248 ppf: 976 ppct: 15 formats: fmt: @attributes: type: P tig: atl: Digital PCR Partition Classification. aug: au: Vynck, Matthijs Yao Chen Gleerup, David Vandesompele, Jo Trypsteen, Wim Lievens, Antoon Thas, Olivier De Spiegelaere, Ward affil: Digital PCR Consortium, Ghent University, Ghent, Belgium sug: ab: BACKGROUND: Partition classification is a critical step in the digital PCR data analysis pipeline. A range of partition classification methods have been developed, many motivated by specific experimental setups. An overview of these partition classification methods is lacking and their comparative properties are often unclear, likely impacting the proper application of these methods. CONTENT: This review provides a summary of all available digital PCR partition classification approaches and the challenges they aim to overcome, serving as a guide for the digital PCR practitioner wishing to apply them. We additionally discuss strengths and weaknesses of these methods, which can further guide practitioners in vigilant application of these existing methods. This review provides method developers with ideas for improving methods or designing new ones. The latter is further stimulated by our identification and discussion of application gaps in the literature, for which there are currently no or few methods available. pubtype: Academic Journal doctype: Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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