Symptom-oriented network pharmacology revealed the mechanism of HuangQi-DanShen herb pair against cerebral ischemia coupled with comprehensive chemical characterization.
In the clinical practice of traditional Chinese medicine, HuangQi-DanShen (HD) is an important drug pair for the treatment of cerebral ischemia (CI). Elucidate the mechanism of HD against CI based on symptom-oriented network pharmacology coupled with comprehensive chemical characterization. UHPLC-Q-...
| Publicado en: | Journal of Ethnopharmacology Vol. 318 |
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| Autores principales: | , , , |
| Formato: | research Journal Article |
| Publicado: |
Elsevier B.V.
Jan2024:Part A
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| Acceso en línea: | Ver este registro en EBSCOhost |
| fields | @attributes: recordID: 1 pdfLink: plink: https://search.ebscohost.com/login.aspx?direct=true&db=ccm&AN=171921075&site=ehost-live header: @attributes: shortDbName: ccm uiTerm: 171921075 longDbName: CINAHL Complete uiTag: AN controlInfo: bkinfo: dissinfo: jinfo: jid: 03788741 3MS jtl: Journal of Ethnopharmacology issn: 03788741 maglogo: N pubinfo: dt: Jan2024:Part A vid: 318 pid: 1004 pub: Elsevier B.V. artinfo: ui: 171921075 171921075 171921075 10.1016/j.jep.2023.116845 171921075 ppct: 1 formats: tig: atl: Symptom-oriented network pharmacology revealed the mechanism of HuangQi-DanShen herb pair against cerebral ischemia coupled with comprehensive chemical characterization. aug: au: Liu, Yuetao Xiang, Ruoxin Lu, Wentian Qin, Xuemei affil: Modern Research Center for Traditional Chinese Medicine, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, No. 92, Wucheng Road, Taiyuan, 030006, Shanxi, PR China sug: subj: Network Pharmacology Drugs, Chinese Herbal Therapeutic Use Medicine, Herbal Plant Extracts Pharmacodynamics Cerebral Ischemia Drug Therapy Chemical Processes Evaluation Medicine, Chinese Traditional Drug Therapy, Combination Epidermal Growth Factor Receptors Matrix Metalloproteinases Molecular Docking Simulation Chromatography, High Pressure Liquid Receptors, Cell Surface Neurotransmitter Agents Gene Expression Dyskinesias Speech Disorders Vision Disorders ab: In the clinical practice of traditional Chinese medicine, HuangQi-DanShen (HD) is an important drug pair for the treatment of cerebral ischemia (CI). Elucidate the mechanism of HD against CI based on symptom-oriented network pharmacology coupled with comprehensive chemical characterization. UHPLC-Q-Exactive Orbitrap-MS technology was firstly used to obtain the chemical profile of HD constituents. A comprehensive strategy combining in-house library, diagnostic ions, Compound Discover software and network databases was then established to identify its chemical constitutes. Symptomatic treatment is a treatment aimed at relieving or eliminating symptoms which is often characterized as a stop-gap measure due to its inability to cure the disease fundamentally. Nevertheless, symptomatic treatment is an indispensable part of clinical practice and has an important place in medical therapeutics. Therefore, network pharmacology technique were used to elucidate molecular mechanisms from the symptoms of CI. Finally, some literatures were further mined to support our conclusions. A total of 190 ingredients were identified in HD. Symptom-oriented network pharmacology analysis indicated that compounds of HD relieved "blood" through the regulation of ADORA2A, ADORA1, PTPN11, MMP9 and EGFR, relieved "qi" via the regulation of ADORA2A, EGFR, MMP9 and CA2. The therapeutic effect of HD on "faint" was linked to PTPN11 and MMP9, while the regulation of "dyskinesia" was related to ADORA2A and EGFR, and ADORA1, PTPN11 and MMP9 were associated withe its effect on "speech disorder". ADORA1, ADORA2A and MMP9 were key to the HD component in treating "visual disturbance". The approach of symptom-oriented network pharmacology coupled with comprehensive chemical characterization proposed a further orientation for exploring the mechanisms of HD against CI. [Display omitted] • Symptom-oriented network pharmacology unveiled mechanisms of HD herb pair against CI. • A strategy for compound characterization of HD to aid network pharmacology analysis. • ADORA1, ADORA2A, PTPN11, CA2, EGFR and MMP9 were essential for HD treatment for CI. pubtype: Academic Journal doctype: research Journal Article ougenre: Article language: English refInfo: holdings: @attributes: islocal: N |
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